FN Archimer Export Format PT J TI Chemiluminescence analysis of the activity of Pecten maximus hemocytes stimulated with zymosan and host-specific Rickettsiales-like organisms BT AF LE GALL, G BACHERE, Evelyne MIALHE, Eric AS 1:1;2:1;3:1; FF 1:;2:;3:; C1 IFREMER,BIOL & ECOL INVERTEBRES MARINS LAB,UNITE RECH PATHOL IMMUNOL & GENET MOLEC,BP 133,F-17390 LA TREMBLADE,FRANCE. C2 IFREMER, FRANCE IN WOS Ifremer jusqu'en 2018 IF 1.556 TC 49 UR https://archimer.ifremer.fr/doc/1991/publication-2759.pdf LA English DT Article AB During a study of Rickettsiales-like organisms (RLO) in Pecten maximus gill, histological observation of free RLO in hemoclymph led us to consider interactions between the pathogen and the hemocytes. Firstly, the production of oxygen radicals by hemocytes was established using a luminol-enhanced chemiluminescence method with zymosan axs stimulant. The effects of serveral inhibitors of oxidative metabolism were investigated. The internalization of RLO into hemocytes was demonstrated by electron microscopy but was not related to chemiluminescent activity. The possible involvement of RLO acid phosphatase as the inhibitor of respiratory burst was assessed. PY 1991 PD OCT SO Diseases Of Aquatic Organisms SN 0177-5103 PU Inter-research VL 11 IS 3 UT A1991GM17600004 BP 181 EP 186 DI 10.3354/dao011181 ID 2759 ER EF