Monoclonal antibody against a gill Rickettsiales-like organism of Pecten maxiumus (Bivalvia): application to indirect immunofluorescence diagnosis

Type Article
Date 1992-12
Language English
Author(s) Le Gall Gyslaine, Mourton Chantal, Boulo VivianeORCID, Paolucci Francis, Pau Bernard, Mialhe Eric
Source Diseases of aquatic organisms (Inter-Research), 1992-12 , Vol. 14 , P. 213-217
Keyword(s) Diagnosis, Rickettsiales like organism, Monoclona antibody, Pecten maximus
Abstract Using hybridoma technology, monoclonal antibodies (MABs) were prepared against a Rickettsiales-like organism (RLO) from the gills of the Saint-Jacques scallop Pecten maximus. The 5D7 MAB was then selected as a specific reagent suitable for an indirect immunofluorescence assay (IIFA) and evaluated for RLO diagnosis on P. maxiumus gill smears. Monoclonal antibody 5D7 showed no cross-reactivity with an RLO associated with a related pectinid species Chlamys opercularis
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Le Gall Gyslaine, Mourton Chantal, Boulo Viviane, Paolucci Francis, Pau Bernard, Mialhe Eric (1992). Monoclonal antibody against a gill Rickettsiales-like organism of Pecten maxiumus (Bivalvia): application to indirect immunofluorescence diagnosis. Diseases of aquatic organisms, 14, 213-217. Open Access version : https://archimer.ifremer.fr/doc/00000/2762/