FN Archimer Export Format
PT J
TI A direct monoclonal antibody sandwich immunoassay for detection of Bonamia ostreae (Ascetospora) in hemolymph samples of the flat oyster Ostrea edulis (Mollusca : Bivalvia)
BT 
AF COCHENNEC, Nathalie
   HERVIO, Dominique
   PANATIER, B
   BOULO, Viviane
   MIALHE, Eric
   ROGIER, H
   GRIZEL, Henri
   PAOLUCCI, F
AS 1:1;2:2;3:3;4:2;5:2;6:4;7:2;8:4;
FF 1:;2:;3:;4:;5:;6:;7:;8:;
C1 Ifremer, La Trinité, France
   IFREMER, BIOL ECOSYST INVERT MARINS LAB, F-17390 LA TREMBLADE, FRANCE.
   SANOFI, F-33501 LIBOURNE, FRANCE.
   SANOFI RECH, F-34082 MONTPELLIER, FRANCE.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   SANOFI, FRANCE
   SANOFI, FRANCE
SI LA TRINITE
IN WOS Ifremer jusqu'en 2018
IF 1.556
TC 18
UR https://archimer.ifremer.fr/doc/1992/publication-2765.pdf
LA English
DT Article
AB Currently recognition of molluscan diseases relies heavily upon light microscopic examination of stained histological sections of molluscan tissues Such histopathological methods are time consuming and relatively inefficient In the study of ongoing epizootics Alternatively, more effluent investigative tools are needed to accelerate disease recognition and quantification Such methods are needed to prevent control and eradicate molluscan diseases This paper reports the development of a direct sandwich enzyme-lmmunosorbent assay (ELISA) for the ascetosporan intrahemocytic parasite Bonamiao ostreae of the flat oyster Ostrea edulis. Bonamiasis is a commercially important epizootic disease of oysters A Bonamia-specific monoclonal antibody is employed in the assay The assay is performed In antibody-coated microplates employing standardized reagent and 50 µl oyster hemolymph samples This procedure was developed into a commercial product, available as a kit Based upon clinical studies, there was a 90 O/o agreement In test results when ELISAs were compared to the reference histopathologic light microscopic examination for the parasites
PY 1992
PD FEB
SO Diseases Of Aquatic Organisms
SN 0177-5103
PU Inter-research
VL 12
IS 2
UT A1992HE94600008
BP 129
EP 134
ID 2765
ER

EF