FN Archimer Export Format
PT J
TI Purification of Rickettsiales-like organisms associated with Pecten maximus (Mollusca : Bivalvia) : serological and biochemical characterization
BT 
AF LE GALL, G
   MIALHE, Eric
AS 1:1;2:1;
FF 1:;2:;
C1 IFREMER,BIOL & ECOL INVERTEBRES MARINS LAB,UNITE RECH & PATHOL IMMUNOL & GENET MOLEC,BP 133,F-17390 LA TREMBLADE,FRANCE.
C2 IFREMER, FRANCE
IN WOS Ifremer jusqu'en 2018
IF 1.556
TC 18
UR https://archimer.ifremer.fr/doc/1992/publication-2769.pdf
LA English
DT Article
AB Significant mortalities have been observed in populations of Saint-Jacques scallops Pecten maximus, in association with numerous intrace1lular colonies of Rickettsiales-like organisms (RLO) in the gi1ls. Isolation of the procaryotes from infected tissues and their subsequent purification were achieved by a specific protocol based on differential and isopycnic centrifugations on sucrose and renografin density gradients. The purity and integrity of purified procaryotes were verified by electron microscopy. Specific polyclonal antibodies were prepared for serological characterization. Fifteen major constituent proteins were electrophoretically identified, their molecular weights ranging between 148 and 16 kD. Ten enzyme activities were biochemica1ly identified, including acid phosphatase and catalase, which could be related to procaryote pathogenicity.
PY 1992
PD APR
SO Diseases Of Aquatic Organisms
SN 0177-5103
PU Inter-research
VL 12
IS 3
UT A1992HU03100008
BP 215
EP 220
ID 2769
ER

EF