FN Archimer Export Format
PT J
TI Needle in a haystack: involvement of the copepod Paracartia grani, in the life-cycle of the oyster pathogen Marteilia refringens
BT 
AF AUDEMARD, Corinne
   LE ROUX, Frédérique
   BARNAUD, Antoine
   COLLINS, Catherine
   SAUTOUR, B
   SAURIAU, Pierre-Guy
   DE MONTAUDOUIN, X
   COUSTAU, C
   COMBES, C
   BERTHE, Franck
AS 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;
FF 1:PDG-DRV-RA-LGP;2:;3:PDG-DRV-RA-SEMII;4:PDG-DRV-RA-GAP;5:;6:;7:;8:;9:;10:PDG-DRV-RA-LGP;
C1 IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
   Univ Bordeaux 1, UMR 5805, Lab Oceanog Biol, CNRS 2, F-33120 Arcachon, France.
   Ctr Rech Ecol Marine & Aquaculture, F-17137 Houmeau, France.
   Univ Perpignan, CBETM, CNRS, UMR 5555 Lab Biol Anim, F-66860 Perpignan, France.
C2 IFREMER, FRANCE
   UNIV BORDEAUX 1, FRANCE
   CTR RECH ECOL MARINE & AQUACULTURE, FRANCE
   UNIV PERPIGNAN, FRANCE
SI LA TREMBLADE
   CAMARET
   LA TREMBLADE RONCE
   LA ROCHELLE
SE PDG-DRV-RA-LGP
   PDG-DRV-RA-SEMII
   PDG-DRV-RA-GAP
   PDG-DRV-RA-CREMA
IN WOS Ifremer jusqu'en 2018
   copubli-univ-france
IF 1.828
TC 66
UR https://archimer.ifremer.fr/doc/2002/publication-2778.pdf
LA English
DT Article
DE ;Experimental transmission;In situ hybridization;PCR;Paracartia grani;Ostrea edulis;Marteilia refringens
AB Marteilia refringens is a major pathogen of the European flat oyster, Ostrea edulis Linnaeus. Since its description, the lifecycle of this protozoan parasite has eluded discovery. Attempts to infect oysters experimentally have been unsuccessful and led to the hypothesis of a complex life-cycle involving several hosts. Knowledge of this life-cycle is of central importance in order to manage oyster disease. However, the exploration of M. refringens life-cycle has been previously limited by the detection tools available and the tremendous number of species to be screened in enzootic areas. In this study, these two restrictions were circumvented by the use of both molecular detection tools and a mesocosm with low biodiversity. Screening of the entire fauna of the pond for AT. refringens DNA was systematically undertaken using PCR. Here, we show that the copepod Paracartia (Acartia) grani is a host of M. refringens. Not only was DNA of M. refringens consistently detectcd in P. grani but also the presence of the parasite in the ovarian tissues was demonstrated using in situ hybridization. Finally, successful experimental transmissions provided evidence that P. grani can be infected from infected flat oysters.
PY 2002
PD SEP
SO Parasitology
SN 0031-1820
PU Cambridge University Press
VL 124
UT 000174925000009
BP 315
EP 323
DI 10.1017/S0031182001001111
ID 2778
ER

EF