FN Archimer Export Format PT J TI AFLP-based genetic linkage maps of the blue mussel (Mytilus edulis) BT AF LALLIAS, Delphine LAPEGUE, Sylvie HECQUET, Celine BOUDRY, Pierre BEAUMONT, A AS 1:2;2:2;3:2;4:2;5:1; FF 1:PDG-DOP-DCN-AGSAE-LGP;2:PDG-DOP-DCN-AGSAE-LGP;3:;4:PDG-DOP-DCB-PFOM-PI;5:; C1 Univ Wales, Coll Nat Sci, Sch Ocean Sci, Bangor LL59 5AB, Gwynedd, Wales. IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France. C2 UNIV WALES, WALES IFREMER, FRANCE SI LA TREMBLADE BREST SE PDG-DOP-DCN-AGSAE-LGP PDG-DOP-DCB-PFOM-PI IN WOS Ifremer jusqu'en 2018 copubli-europe IF 2.64 TC 25 UR https://archimer.ifremer.fr/doc/2007/publication-2830.pdf LA English DT Article DE ;Mytilus edulis;Genetic linkage map;Blue mussel;AFLP AB We report the construction of the first genetic linkage map in the blue mussel, Mytilus edulis. AFLP markers were used in 86 full-sib progeny from a controlled pair mating, applying a double pseudo-test cross strategy. Thirty-six primer pairs generated 2354 peaks, of which 791 (33.6%) were polymorphic in the mapping family. Among those, 341 segregated through the female parent, 296 through the male parent (type 1:1) and 154 through both parents (type 3:1). Chi-square goodness-of-fit tests revealed that 71% and 73% of type 1:1 and 3:1 markers respectively segregated according to Mendelian inheritance. Sex-specific linkage maps were built with mapmaker 3.0 software. The female framework map consisted of 121 markers ordered into 14 linkage groups, spanning 862.8 cM, with an average marker spacing of 8.0 cM. The male framework map consisted of 116 markers ordered into 14 linkage groups, spanning 825.2 cM, with an average marker spacing of 8.09 cM. Genome coverage was estimated to be 76.7% and 75.9% for the female and male framework maps respectively, rising to 85.8% (female) and 86.2% (male) when associated markers were included. Twelve probable homologous linkage group pairs were identified and a consensus map was built for nine of these homologous pairs based on multiple and parallel linkages of 3:1 markers, spanning 816 cM, with joinmap 4.0 software. PY 2007 PD AUG SO Animal Genetics SN 0268-9146 PU Blackwell science VL 38 IS 4 UT 000248335900003 BP 340 EP 349 DI 10.1111/j.1365-2052.2007.01611.x ID 2830 ER EF