FN Archimer Export Format
PT J
TI Effects of cytochalasin B on fertilization and ploidy in the Pacific oyster Crassostrea gigas
BT 
AF LEDU, Christophe
   MCCOMBIE, Helen
AS 1:;2:;
FF 1:PDG-DRV-RA-DCOP-AT;2:PDG-DRV-RA-LGP;
C1 Inst Francais Rech Exploitat Mer, IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
C2 IFREMER, FRANCE
SI TAHITI
   LA TREMBLADE
SE PDG-DRV-RA-DCOP-AT
   PDG-DRV-RA-LGP
IN WOS Ifremer jusqu'en 2018
IF 0.32
TC 5
UR https://archimer.ifremer.fr/doc/2003/publication-2845.pdf
LA English
DT Article
DE ;Crassostrea gigas;Fertilization;Cytochalasin B;Polar body;Polyploidy
AB The effects of cytochalasin B on fertilization and ploidy in Crassostrea gigas were examined in a controlled experiment. This enabled us to better understand why variable ploidy results have been found with the retention of polar bodies in previous studies on bivalve mollusks, and what the interaction with fertilization may be. By looking at the early development of Crassostrea gigas embryos with sequential samples stained using Hoechst 33258, a fluorescent DNA-specific dye, we compared normal evolution of fertilized eggs with that obtained with cytochalasin B treatment. In treated eggs, embryos with both three and four pronuclei were found after the second meiotic division. When embryos had three pronuclei, these were identified as the female pronucleus plus two chromatin groups arising from the retention of the two polar bodies. The fourth pronucleus present in some embryos was the male pronucleus. The absence of the male pronucleus in the embryos with only three pronuclei was interpreted as gynogenetic development due to the inhibitory effects of cytochalasin B on penetration of the sperm into the eggs. This result explains the findings of several previous studies where different ploidies resulted from double polar body inhibition in bivalve species. Implications of such treatments for post-embryogenic development are discussed.
PY 2003
PD SEP
SO Invertebrate reproduction and development
SN 0168-8170
PU Balaban Publishers
VL 44
IS 2-3
UT 000187231700006
BP 131
EP 137
ID 2845
ER

EF