FN Archimer Export Format
PT J
TI Detection of ostreid herpesvirus 1 (OsHV-1) DNA in seawater by PCR: influence of water parameters in bioassays
BT 
AF VIGNERON, Vassilia
   SOLLIEC, Gaelle
   MONTANIE, Hélène
   RENAULT, Tristan
AS 1:;2:;3:;4:;
FF 1:;2:PDG-DOP-DCN-AGSAE-LGP;3:;4:PDG-DOP-DCN-AGSAE-LGP;
C1 IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
   Univ La Rochelle, Lab Biol & Environm Marins, F-17042 La Rochelle, France.
C2 IFREMER, FRANCE
   UNIV LA ROCHELLE, FRANCE
SI LA TREMBLADE
SE PDG-DOP-DCN-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
   copubli-univ-france
IF 1.583
TC 24
UR https://archimer.ifremer.fr/doc/2004/publication-2909.pdf
LA English
DT Article
DE ;Temperature;PCR;Detection;Seawater;Viral DNA;Oyster herpesvirus 1
AB Since 1991, herpesvirus infections have been reported among larvae and juveniles of various bivalves. Most of the studies focused on detection of viral infections of economically important species. However, the persistence of bivalve herpesviruses; in the marine environment is poorly documented. The present study concerns the role of seawater parameters in Ostreid Herpesvirus I (OsHV-1) detection by polymerase chain reaction (PCR). Viral DNA extracted from purified particles or virions present in infected oyster larvae were detected by PCR after storage in different media at different temperatures. The lowest detection threshold was found using distilled water or Tris EDTA buffer. In seawater, the threshold was higher. The use of sterile media permitted detection of viral DNA stored over a longer period. Storage temperature also had a significant influence on detection, with lower temperatures promoting DNA detection over a longer period. In summary, water parameters such as temperature influenced detection of OsHV-1 DNA by PCR. However, the PCR technique may also be successfully applied to samples in natural seawater. Indeed, the PCR technique permitted detection of naked viral DNA at 100 ng l(-1) in seawater in bioassays.
PY 2004
PD NOV
SO Diseases of aquatic organisms
SN 0177-5103
PU Inter-Research
VL 62
IS 1-2
UT 000226680900004
BP 35
EP 44
ID 2909
ER

EF