FN Archimer Export Format
PT J
TI Two Vibrio splendidus related strains collaborate to kill Crassostrea gigas: taxonomy and host alterations
BT 
AF GAY, Melanie
   RENAULT, Tristan
   PONS, Anne-Marie
   LE ROUX, Frédérique
AS 1:;2:;3:;4:;
FF 1:PDG-DOP-DCN-AGSAE-LGP;2:PDG-DOP-DCN-AGSAE-LGP;3:;4:;
C1 IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
   Univ La Rochelle, Lab Biotechnol & Chim Bioorgan, CNRS, FRE 2766, F-17042 La Rochelle, France.
C2 IFREMER, FRANCE
   UNIV LA ROCHELLE, FRANCE
SI LA TREMBLADE
SE PDG-DOP-DCN-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
   copubli-univ-france
IF 1.583
TC 105
UR https://archimer.ifremer.fr/doc/2004/publication-2912.pdf
LA English
DT Article
DE ;Histopathology;Molecular taxonomy;Agonism;Vibriosis
AB For several years, strains phenotypically related to Vibrio splendidus have been associated with mortality outbreaks of molluscs. A former study on Crassostrea gigas demonstrated the genetic diversity of V splendidus strains associated with diseased animals. Another study suggested that different strains may act in an additive/synergistic way leading to higher C. gigas mortality rates. Here, a strain pair (31+32) was characterised at taxonomic and virulence levels. Using a polyphasic approach, these strains were confirmed to be V splendidus-related, without a clear discrimination between V. kanaloae and V. pomeroyi since hybridisation rates with both these strains were above 70%. Following experimental infection of C. gigas by injection in the adductor muscle or in the pallial cavity, the host alterations induced were described. After injection of strains 31 and/or 32, bacteria were localised at the periphery of the muscle and induced extensive lesions of the translucent part of the adductor muscle. Muscle alterations were of 3 kinds: (1) presence of isolated rounded muscular fibres containing non-homogenous granular material and surrounded by a translucent halo; (2) presence of non-homogenous granular material in the cytoplasm of entire muscle bands; (3) affection of wide muscle areas with extremely condensed muscle fibres. Infiltration associated with these lesions was notably absent in the vast majority of the individuals.
PY 2004
PD NOV
SO Diseases of aquatic organisms
SN 0177-5103
PU Inter-Research
VL 62
IS 1-2
UT 000226680900007
BP 65
EP 74
ID 2912
ER

EF