FN Archimer Export Format
PT J
TI Detection of ostreid herpesvirus-1 (OsHV-1) by PCR using a rapid and simple method of DNA extraction from oyster larvae
BT 
AF BATISTA, Frederico
   TARIS, Nicolas
   BOUDRY, Pierre
   RENAULT, Tristan
AS 1:1,2;2:2;3:2;4:2;
FF 1:PDG-DOP-DCN-AGSAE-LGP;2:PDG-DOP-DCN-AGSAE-LGP;3:PDG-DOP-DCN-AGSAE-LGP;4:PDG-DOP-DCN-AGSAE-LGP;
C1 INIAP, IPIMAR, CRIPSul, P-8700305 Olhao, Portugal.
   IFREMER, LGP, F-17390 La Tremblade, France.
C2 IPIMAR, PORTUGAL
   IFREMER, FRANCE
SI LA TREMBLADE
SE PDG-DOP-DCN-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
   copubli-europe
IF 1.361
TC 7
UR https://archimer.ifremer.fr/doc/2005/publication-2916.pdf
LA English
DT Article
DE ;DNA extraction;Detection;Larvae;Oyster;OsHV 1;Herpesvirus
AB A DNA extraction procedure was developed for the detection of ostreid herpesvirus-1 (OsHV-1) using the polymerase chain reaction (PCR) in oyster larvae. The DNA extraction procedure developed was tested on 8 larval samples. Abnormal nuclei with characteristic features associated with OsHV-1 infections were only observed in samples in which the viral DNA was detected by PCR. A previously described competitive PCR method was applied to detect inhibition during PCR reactions. The results show that the method can be used on small amounts of oyster larvae (3 mg) for the detection of OsHV-1 DNA by PCR.
PY 2005
PD APR
SO Diseases of aquatic organisms
SN 0177-5103
PU Inter-Research
VL 64
IS 1
UT 000229606200001
BP 1
EP 4
ID 2916
ER

EF