FN Archimer Export Format
PT J
TI Neoplasia detection in Macoma balthica from the Gulf of Gdansk: comparison of flow cytometry, histology and chromosome analysis
BT 
AF SMOLARZ, Kasia
   RENAULT, Tristan
   SOLETCHNIK, Patrick
   WOLOWICZ, Macciek
AS 1:1,3;2:1;3:2;4:3;
FF 1:PDG-DOP-DCN-AGSAE-LGP;2:PDG-DOP-DCN-AGSAE-LGP;3:PDG-DOP-LER-LERPC;4:;
C1 IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
   IFREMER, Lab Conchylicole Poitou Charentes, F-17390 La Tremblade, France.
   Univ Gdansk, Inst Oceanog, Lab Estuarine Ecol, PL-81378 Gdynia, Poland.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   UNIV GDANSK, POLAND
SI LA TREMBLADE
SE PDG-DOP-DCN-AGSAE-LGP
   PDG-DOP-LER-LERPC
IN WOS Ifremer jusqu'en 2018
   copubli-europe
IF 1.361
TC 10
UR https://archimer.ifremer.fr/doc/2005/publication-2918.pdf
LA English
DT Article
DE ;Baltic Sea;Macoma balthica;Neoplasia;Cytogenetics;Histology;Flow cytometry
AB A flow cytometry protocol was applied for the detection of neoplasia in Macoma balthica L. from the Gulf of Gdansk (Baltic Sea, Poland). A simple method, based on an osmotic shock, was used to permeabilise gill cells. The cytometric pattern of normal clams consisted of 2 peaks, a major peak B and a smaller peak C. The cytometric pattern of affected clams consisted of 2 peaks named B' and C'. Two parameters were used to define the stages of abnormalities in M. balthica clams based on the percentage of cells in peaks B, C, B' and C' and on the ratio between the fluorescence value of peaks B, C, B' and C' in all individuals. Three stages of neoplasia were clearly distinguished by flow cytometry considering peak C'. Stage 1 was characterised by a major population of cells in peak B' and more than 10 % of cells in the C' peak. Stage 2 consisted of a lower percentage of cells in peak B' and more than 25 % of cells in peak C'. Stage 3 of the neoplasia was characterised by a further reduction in peak B' and more than 40 % of cells in peak C'. Flow cytometry allowed for objective detection of neoplasia and provided a rapid method for measuring the DNA content of thousands of cells per individual. The accuracy of flow cytometry was assessed by comparing with standard histological techniques, used here as a reference technique for the detection of neoplasia, and with chromosome analysis. All individuals were analysed in parallel using the 3 techniques. The proportion of normal and affected individuals diagnosed using flow cytometry was comparable to the proportion determined by histology and chromosome analysis.
PY 2005
PD JUN
SO Diseases of aquatic organisms
SN 0177-5103
PU Inter-Research
VL 65
IS 3
UT 000231965000002
BP 187
EP 195
ID 2918
ER

EF