|Author(s)||Smolarz Kasia1, 3, Renault Tristan1, Soletchnik Patrick2, Wolowicz Macciek3|
|Affiliation(s)||1 : IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
2 : IFREMER, Lab Conchylicole Poitou Charentes, F-17390 La Tremblade, France.
3 : Univ Gdansk, Inst Oceanog, Lab Estuarine Ecol, PL-81378 Gdynia, Poland.
|Source||Diseases of aquatic organisms (0177-5103) (Inter-Research), 2005-08 , Vol. 66 , N. 1 , P. 41-46|
|WOS© Times Cited||8|
|Keyword(s)||Prevalence, Macoma balthica, Flow cytometry, Gill neoplasia|
|Abstract||Using flow cytometry, 234 Macoma balthica were examined during a survey to determine frequency of neoplasia in the Gulf of Gdansk (Poland). Clams were collected in 4 locations and DNA content in gill tissue cells was determined by flow cytometry using propidium iodide staining. Cell permeabilization was induced by osmotic shock. Prevalence of neoplasia ranged from 9.6 to 26.7 % depending on location. DNA content in aneuploid cells was higher than in normal dividing cells. The fluorescence value for aneuploid cells corresponded to tetraploid/pentaploid cells. Three stages of neoplasia were defined, based on the percentage of aneuploid cells determined by flow cytometry. Histopathological and cytogenetic analyses were also carried out on the same clams for comparative study. Proportions of normal and affected clams detected using flow cytometry were similar to those identified using both methods. In the present study, no clear relationship was demonstrated between prevalence of neoplasia and pollutant detection in the different sampling sites.|
Smolarz Kasia, Renault Tristan, Soletchnik Patrick, Wolowicz Macciek (2005). Survey for neoplasia in Macoma balthica from the Gulf of Gdansk by flow cytometry. Diseases of aquatic organisms, 66(1), 41-46. Publisher's official version : https://doi.org/10.3354/dao066041 , Open Access version : https://archimer.ifremer.fr/doc/00000/2919/