FN Archimer Export Format
PT J
TI Survey for neoplasia in Macoma balthica from the Gulf of Gdansk by flow cytometry
BT 
AF SMOLARZ, Kasia
   RENAULT, Tristan
   SOLETCHNIK, Patrick
   WOLOWICZ, Macciek
AS 1:1,3;2:1;3:2;4:3;
FF 1:PDG-DOP-DCN-AGSAE-LGP;2:PDG-DOP-DCN-AGSAE-LGP;3:PDG-DOP-LER-LERPC;4:;
C1 IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
   IFREMER, Lab Conchylicole Poitou Charentes, F-17390 La Tremblade, France.
   Univ Gdansk, Inst Oceanog, Lab Estuarine Ecol, PL-81378 Gdynia, Poland.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   UNIV GDANSK, POLAND
SI LA TREMBLADE
SE PDG-DOP-DCN-AGSAE-LGP
   PDG-DOP-LER-LERPC
IN WOS Ifremer jusqu'en 2018
   copubli-europe
IF 1.361
TC 8
UR https://archimer.ifremer.fr/doc/2005/publication-2919.pdf
LA English
DT Article
DE ;Prevalence;Macoma balthica;Flow cytometry;Gill neoplasia
AB Using flow cytometry, 234 Macoma balthica were examined during a survey to determine frequency of neoplasia in the Gulf of Gdansk (Poland). Clams were collected in 4 locations and DNA content in gill tissue cells was determined by flow cytometry using propidium iodide staining. Cell permeabilization was induced by osmotic shock. Prevalence of neoplasia ranged from 9.6 to 26.7 % depending on location. DNA content in aneuploid cells was higher than in normal dividing cells. The fluorescence value for aneuploid cells corresponded to tetraploid/pentaploid cells. Three stages of neoplasia were defined, based on the percentage of aneuploid cells determined by flow cytometry. Histopathological and cytogenetic analyses were also carried out on the same clams for comparative study. Proportions of normal and affected clams detected using flow cytometry were similar to those identified using both methods. In the present study, no clear relationship was demonstrated between prevalence of neoplasia and pollutant detection in the different sampling sites.
PY 2005
PD AUG
SO Diseases of aquatic organisms
SN 0177-5103
PU Inter-Research
VL 66
IS 1
UT 000232577300007
BP 41
EP 46
DI 10.3354/dao066041
ID 2919
ER

EF