FN Archimer Export Format
PT J
TI Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro
BT 
AF HELLIO, Claire
   BADO NILLES, Anne
   GAGNAIRE, Beatrice
   RENAULT, Tristan
   THOMAS-GUYON, Helene
AS 1:1;2:2;3:3;4:3;5:2;
FF 1:;2:;3:PDG-DOP-DCN-AGSAE-LGP;4:PDG-DOP-DCN-AGSAE-LGP;5:;
C1 Univ La Rochelle, Ctr Rech Ecosyst Littoraux Anthropises, UMR6217, CBRS IFREMER, F-17042 La Rochelle 01, France.
   Univ Portsmouth, Sch Biol Sci, Portsmouth PO1 2DY, Hants, England.
   IFREMER, LGP, F-17390 La Tremblade, France.
C2 UNIV LA ROCHELLE, FRANCE
   UNIV PORTSMOUTH, ENGLAND
   IFREMER, FRANCE
SI LA TREMBLADE
   UNIV-FRANCAISE
SE PDG-DOP-DCN-AGSAE-LGP
   PDG-DOP-DCN-AGSAE-CRELA
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 3.16
TC 83
UR https://archimer.ifremer.fr/doc/2007/publication-2926.pdf
LA English
DT Article
DE ;Prophenoloxidase;Phenoloxidase;Oyster;Immunity;Defence;Crassostrea gigas
AB The prophenoloxidase (ProPO) system is the origin of melanin production and is considered to be an innate defence mechanism in invertebrates. In different bivalve species, phenoloxidase (PO) is present in the haemolymph as an inactive form of ProPO. The present study focuses on the Pacific adult oyster, Crassostrea gigas, an economically important bivalve species along French coasts. The results indicate that many factors may inhibit the PO-like activity. These include: phenylthiourea (PTU), sodium diethylthiocarbamate (DETC), beta-mercaptoethanol and tropolone, which repressed the spontaneous PO activity. The activation of PO-like activity in C. gigas acellular fraction by lipopolysaccharide (LPS) involved participation of other factors, including at least one serine protease. PO was present as proPO in the acellular fraction of haemolymph and haemocytes of C. gigas and could be activated by an exogenous protease (trypsin-N-tosyl-L-phenylalanine chloromethyl ketone) when used at 1 g L-1. Treatment of acellular fractions with other modulators/activators namely LPS (1 g L-1), zymosan (0.6 g L-1) or laminarin (0.6 g L-1) also increased PO-like activity but to a less important way. The study demonstrated the evidence of a true phenoloxidase activity in Pacific oyster, C. gigas (Thunberg). The activation of a proPO system by non-self molecules suggests the role played by PO in vivo in the internal defence mechanisms. Understanding the activation of the ProPO system could enable the evaluation of the health of oyster stocks. (c) 2006 Published by Elsevier Ltd.
PY 2007
PD APR
SO Fish & Shellfish Immunology
SN 1050-4648
PU Elsevier
VL 22
IS 4
UT 000244247700014
BP 433
EP 440
DI 10.1016/j.fsi.2006.06.014
ID 2926
ER

EF