Determination of peptaibol trace amounts in marine sediments by liquid chromatography/electrospray ionization-ion trap-mass spectrometry
|Author(s)||Poirier Laurence1, Amiard Jean-Claude1, Mondeguer Florence2, Quiniou Francoise3, Ruiz Nicolas1, Pouchus Yves Francois1, Montagu Monique1|
|Affiliation(s)||1 : Univ Nantes, Nantes Atlantique Univ, SMAB EA2160, Fac Pharm, F-44000 Nantes, France.
2 : IFREMER, DEL PN, Ctr Nantes, F-44311 Nantes 3, France.
3 : IFREMER, Ctr Brest, BE EX, F-29280 Plouzane, France.
|Source||Journal of Chromatography A (0021-9673) (Elsevier), 2007-08 , Vol. 1160 , N. 1-2 , P. 106-113|
|WOS© Times Cited||9|
|Keyword(s)||Matched calibration, Matrix, Electrospray ionization ion trap mass spectrometry ESI IT MS, Marine fungal contamination, Trichoderma sp., Fungal peptide metabolites|
|Abstract||Extraction followed by reverse phase liquid chromatography (LC)/electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS) analysis has been successfully developed for the determination of peptaibols, fungal toxic metabolites, in marine sediments. Spiking experiments showed that the mean recovery of target compounds exceeded 85% at a spiking level of 10 ng/g of sediment (wet weight). Detection and quantification limits were 250 and 830 pg/g of sediment, respectively. The method developed constituted the first sensitive assay for quantification of peptaibol trace amounts in a natural environment. A concentration of 5 ng/g in sediment samples collected from Fier d'Ars was found.