Determination of peptaibol trace amounts in marine sediments by liquid chromatography/electrospray ionization-ion trap-mass spectrometry
| Type | Article | ||||||||
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| Date | 2007-08 | ||||||||
| Language | English | ||||||||
| Author(s) | Poirier Laurence1, Amiard Jean-Claude1, Mondeguer Florence 2, Quiniou Francoise3, Ruiz Nicolas1, Pouchus Yves Francois1, Montagu Monique1 |
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| Affiliation(s) | 1 : Univ Nantes, Nantes Atlantique Univ, SMAB EA2160, Fac Pharm, F-44000 Nantes, France. 2 : IFREMER, DEL PN, Ctr Nantes, F-44311 Nantes 3, France. 3 : IFREMER, Ctr Brest, BE EX, F-29280 Plouzane, France. |
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| Source | Journal of Chromatography A (0021-9673) (Elsevier), 2007-08 , Vol. 1160 , N. 1-2 , P. 106-113 | ||||||||
| DOI | 10.1016/j.chroma.2007.04.006 | ||||||||
| WOS© Times Cited | 10 | ||||||||
| Keyword(s) | Matched calibration, Matrix, Electrospray ionization ion trap mass spectrometry ESI IT MS, Marine fungal contamination, Trichoderma sp., Fungal peptide metabolites | ||||||||
| Abstract | Extraction followed by reverse phase liquid chromatography (LC)/electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS) analysis has been successfully developed for the determination of peptaibols, fungal toxic metabolites, in marine sediments. Spiking experiments showed that the mean recovery of target compounds exceeded 85% at a spiking level of 10 ng/g of sediment (wet weight). Detection and quantification limits were 250 and 830 pg/g of sediment, respectively. The method developed constituted the first sensitive assay for quantification of peptaibol trace amounts in a natural environment. A concentration of 5 ng/g in sediment samples collected from Fier d'Ars was found. |
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