TY - JOUR T1 - Primers and polymerase chain reaction conditions for DNA barcoding teleost fish based on the mitochondrial cytochrome b and nuclear rhodopsin genes A1 - Sevilla,Rafael G. A1 - Diez,Amalia A1 - Noren,Michael A1 - Mouchel,Olivier A1 - Jerome,Marc A1 - Verrez-Bagnis,Veronique A1 - Van Pelt,Hilde A1 - Favre Krey,Laurence A1 - Krey,Grigorios A1 - Bautista,José M. AD - Univ Complutense Madrid, Dept Biochem & Mol Biol 4, Fac Vet, E-28040 Madrid, Spain. AD - Swedish Museum Nat Hist, SE-10405 Stockholm, Sweden. AD - French Res Inst Exploitat Sea IFREMER, F-44311 Nantes, France. AD - Netherlands Inst Fisheries Res, NL-1970 AB Ijmuiden, Netherlands. AD - Natl Agr Res Fdn Fisheries Res Inst, GR-64007 Nea Peramos, Kavala, Greece. UR - https://archimer.ifremer.fr/doc/00000/3034/ DO - 10.1111/j.1471-8286.2007.01863.x KW - Teleost KW - Rhodopsin KW - PCR primers KW - Mitochondrial cytochrome b KW - Fish KW - DNA barcoding N2 - This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according to their mitochondrial cytochrome b and nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine fish species comprising the main Actinopterygii family groups. When used in phylogenetic analyses, its combination of two genes with different evolutionary rates serves to identify fish at the species level. We provide a flow diagram indicating our validated polymerase chain reaction amplification conditions for barcoding and species identification applications as well as population structure or haplotyping analyses, adaptable to high-throughput analyses. Y1 - 2007/09 PB - Blackwell science JF - Molecular Ecology Notes SN - 1471-8278 VL - 7 IS - 5 SP - 730 EP - 734 ID - 3034 ER -