FN Archimer Export Format
PT J
TI Primers and polymerase chain reaction conditions for DNA barcoding teleost fish based on the mitochondrial cytochrome b and nuclear rhodopsin genes
BT 
AF SEVILLA, Rafael G.
   DIEZ, Amalia
   NOREN, Michael
   MOUCHEL, Olivier
   JEROME, Marc
   VERREZ-BAGNIS, Veronique
   VAN PELT, Hilde
   FAVRE KREY, Laurence
   KREY, Grigorios
   BAUTISTA, José M.
AS 1:1;2:1;3:2;4:3;5:3;6:3;7:4;8:5;9:5;10:1;
FF 1:;2:;3:;4:PDG-DOP-DCN-STAM;5:PDG-DOP-DCN-STAM;6:PDG-DOP-DCN-STAM;7:;8:;9:;10:;
C1 Univ Complutense Madrid, Dept Biochem & Mol Biol 4, Fac Vet, E-28040 Madrid, Spain.
   Swedish Museum Nat Hist, SE-10405 Stockholm, Sweden.
   French Res Inst Exploitat Sea IFREMER, F-44311 Nantes, France.
   Netherlands Inst Fisheries Res, NL-1970 AB Ijmuiden, Netherlands.
   Natl Agr Res Fdn Fisheries Res Inst, GR-64007 Nea Peramos, Kavala, Greece.
C2 UNIV COMPLUTENSE MADRID, SPAIN
   MNHN, SWEDEN
   IFREMER, FRANCE
   RIVO, NETHERLANDS
   NAGREF, GREECE
SI NANTES
SE PDG-DOP-DCN-STAM
IN WOS Ifremer jusqu'en 2018
   copubli-europe
IF 1.257
TC 146
UR https://archimer.ifremer.fr/doc/2007/publication-3034.pdf
LA English
DT Article
DE ;Teleost;Rhodopsin;PCR primers;Mitochondrial cytochrome b;Fish;DNA barcoding
AB This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according to their mitochondrial cytochrome b and nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine fish species comprising the main Actinopterygii family groups. When used in phylogenetic analyses, its combination of two genes with different evolutionary rates serves to identify fish at the species level. We provide a flow diagram indicating our validated polymerase chain reaction amplification conditions for barcoding and species identification applications as well as population structure or haplotyping analyses, adaptable to high-throughput analyses.
PY 2007
PD SEP
SO Molecular Ecology Notes
SN 1471-8278
PU Blackwell science
VL 7
IS 5
UT 000249193000002
BP 730
EP 734
DI 10.1111/j.1471-8286.2007.01863.x
ID 3034
ER

EF