FN Archimer Export Format
PT J
TI Genetic polymorphism of glutamine synthetase and delta-9 desaturase in families of Pacific oyster Crassostrea gigas and susceptibility to summer mortality
BT 
AF DAVID, Elise
   BOUDRY, Pierre
   DEGREMONT, Lionel
   TANGUY, Amelie
   QUERE, N
   SAMAIN, Jean-Francois
   MORAGA, D
AS 1:1;2:2;3:2,3;4:4;5:1,4;6:5;7:1;
FF 1:;2:PDG-DOP-DCN-AGSAE-LGP;3:PDG-DOP-DCN-AGSAE-LGP;4:;5:;6:PDG-DOP-DCB-PFOM-PI;7:;
C1 Univ Bretagne Occidentale, Inst Univ Europeen Mer, LEMAR, CNRS,UMR 6539, F-29280 Plouzane, France.
   IFREMER, Ctr La Tremblade, Lab Genet & Pathol, F-17390 La Tremblade, France.
   Virginia Inst Marine Sci, Aquaculture Genet & Breeding Technol Ctr, Gloucester Point, VA 23062 USA.
   Lab Adaptat & Diversite Milieu Marin, UMR 7144, Biol Stn, F-29682 Roscoff, France.
   IFREMER, Ctr Brest, UMR Physiol & Ecophysiol Mollusques Marins, F-29280 Plouzane, France.
C2 UBO, FRANCE
   IFREMER, FRANCE
   VIRGINIA INST MARINE SCI, USA
   UNIV PARIS 06, FRANCE
   IFREMER, FRANCE
SI LA TREMBLADE
   BREST
SE PDG-DOP-DCN-AGSAE-LGP
   PDG-DOP-DCB-PFOM-PI
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 1.75
TC 8
UR https://archimer.ifremer.fr/doc/2007/publication-3040.pdf
LA English
DT Article
DE ;SSCP;Mortality;Glutamine synthetase;Delta 9 desaturase;Crassostrea gigas
AB Large-scale mortality events have been observed in Pacific oyster Crassostrea gigas on the west coast of France since the early 1980s, particularly during summer. In order to understand the causes of this mortality, two generations of oysters from single-pair matings were studied in three sites on the French Atlantic coast (Baie-des-Veys, Auray and Ronce-les-Bains). The present paper examines the role of two candidate genes in the susceptibility of oysters to summer mortality, and the selective pressure exerted by such mortality on their polymorphism. Glutamine synthetase (amino-acid metabolism) and delta-9 desaturase (lipid metabolism) genes were studied in the successive generations, using polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP). Observed and expected genotypic frequencies were compared. Three different alleles were detected for the glutamine synthetase gene and two for delta-9 desaturase. Allele C of glutamine synthetase seemed to be counter-selected in some second generation families. Allele B of delta-9 desaturase gene was potentially counter-selected at Auray in the families showing higher mortality, and strong selection against 1313 homozygotes was observed. These observations led us to conclude that any selective effect of summer mortality on allele C of glutamine synthetase gene or allele B of delta-9 desaturase gene could be mediated either directly or via linkage to other loci involved in physiological pathways affecting susceptibility. (C) 2007 Elsevier B.V. All rights reserved.
PY 2007
PD OCT
SO Journal of Experimental Marine Biology and Ecology
SN 0022-0981
PU Elsevier
VL 349
IS 2
UT 000249346400007
BP 272
EP 283
DI 10.1016/j.jembe.2007.05.016
ID 3040
ER

EF