FN Archimer Export Format
PT J
TI In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes
BT 
AF LAMBERT, Christophe
   SOUDANT, Philippe
   JEGADEN, Marine
   DELAPORTE, Maryse
   LABREUCHE, Yannick
   MOAL, Jeanne
   SAMAIN, Jean-Francois
AS 1:1;2:1;3:1;4:2;5:2;6:;7:2;
FF 1:;2:;3:;4:PDG-DOP-DCB-PFOM-PI;5:PDG-DOP-DCB-PFOM-PI;6:PDG-DOP-DCB-PFOM-PI;7:PDG-DOP-DCB-PFOM-PI;
C1 Univ Bretagne Occidentale, Inst Univ Europeeen Mer, Lab Sci Environm Marin, F-29280 Plouzane, France.
   IFREMER, Ctr Brest, UMR 100, F-29280 Plouzane, France.
C2 UBO, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-DOP-DCB-PFOM-PI
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 1.735
TC 43
UR https://archimer.ifremer.fr/doc/2007/publication-3057.pdf
LA English
DT Article
DE ;NO synthase;NADPH oxidase;Flow cytometry;Reactive nitrogen intermediate RNI;Reactive oxygen intermediate ROI;Hemocytes;Crassostrea gigas
AB Bivalve hemocyte competence has been measured by quantifying functional characteristics, including reactive oxygen intermediate (ROI) production after activation with zymosan or phorbol myristate acetate (PMA). However, untreated oyster hemocytes also produce ROI and RNI (reactive nitrogen intermediates) after bleeding even if not stimulated by Zymosan or PMA. Extensive investigation of this parameter by flow cytometry showed that, in vitro, ROI/RNI production by untreated hemocytes maintained in seawater appeared to be independent of both bacterial burden in the serum and non-self particle phagocytosis. ROI/ RNI production in granulocytes was higher than in hyalinocytes and could be intensified when activated by zymosan but not by PMA. Both cell types used NADPH-oxidase- and NO-synthase-like pathways to produce these molecules; the NO-synthase pathway seemed relatively more dominant in hyalinocytes and NADPH-oxidase appeared more effective in granulocytes. These results provide new insights for interpreting the modulation of ROI/RNl production by untreated hemocytes shown by other studies, relative to environmental conditions or physiological status of the oysters. (c) 2007 Elsevier B.V. All rights reserved.
PY 2007
PD SEP
SO Aquaculture
SN 0044-8486
PU Elsevier
VL 270
IS 1-4
UT 000249448300041
BP 413
EP 421
DI 10.1016/j.aquaculture.2007.04.074
ID 3057
ER

EF