FN Archimer Export Format
PT J
TI Diatom artificial medium (DAM) a new artificial medium for the diatom Haslea ostrearia and other marine microalgae
BT 
AF GAGNEUX MOREAUX, Sindy
   MOREAU, Christophe
   GONZALEZ, Jean-Louis
   COSSON, Richard P.
AS 1:1;2:1;3:2;4:1;
FF 1:;2:;3:PDG-DOP-DCN-BE-LBCM;4:;
C1 Univ Nantes, ISOMer, EA2663, F-44322 Nantes 3, France.
   IFREMER, F-83507 La Seyne Sur Mer, France.
C2 UNIV NANTES, FRANCE
   IFREMER, FRANCE
SI TOULON
SE PDG-DOP-DCN-BE-LBCM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 0.788
TC 19
UR https://archimer.ifremer.fr/doc/2007/publication-3075.pdf
LA English
DT Article
DE ;Metal speciation;Biochemical parameters;Diatom;Microalgae;Artificial culture medium
AB Artificial media are used in physiological studies of microalgae to maintain consistent conditions from one experiment to another and these media must be adapted to the needs of the organism studied. The artificial medium, in this case named diatom artificial medium (DAM), was designed to maintain long-term cultures of Haslea ostrearia and 19 other planktonic microalgae, and to allow physiological studies related to metal metabolism. The biomass and biochemical composition of H. ostrearia grown in the DAM and in a modified Provasoli medium were compared to assess the suitability of this new artificial medium for the culture of this diatom. The DAM provided sufficient nutrients to allow H. ostrearia to grow as efficiently as in the enriched seawater medium, without negative impact on metabolism. The DAM was tested with 19 other microalgae in order to widen its potential use, and 18 of the 19 showed a good adaptation to this medium. The chemical speciation of metals (Cd, Cu, Pb, Zn) was assessed using a speciation mathematical model. The presence of EDTA resulted in the total complexation of the trace metals implying that they were present in a sole chemical species in the DAM.
PY 2007
PD OCT
SO Journal of Applied Phycology
SN 0921-8971
PU Springer
VL 19
IS 5
UT 000249205500022
BP 549
EP 556
DI 10.1007/s10811-007-9169-4
ID 3075
ER

EF