FN Archimer Export Format PT J TI Cell separation by centrifugal elutriation BT AF BACHERE, Evelyne CHAGOT, Dominique GRIZEL, Henri AS 1:;2:;3:; FF 1:PDG-DOP-DCM-BOME;2:;3:PDG-DOP-DCM-SREU; SI PALAVAS LA REUNION SE PDG-DOP-DCM-BOME PDG-DOP-DCM-SREU TC 0 UR https://archimer.ifremer.fr/doc/1988/publication-3118.pdf LA English DT Article DE ;Bivalve mollusc;Centrifugal elutriation;Cell separation AB There is an ongoing need to isolate and purify ceIls in bivalve mollusc disease research. Many separation techniques differentiate cells solely by a single factor and usually require additives in the medium that may damage live cells. Centrifugai elutriation is a technique that separates particles by size and density simultaneously and can be conducted in physiological media without additives. Living cells can be separated without chemical damage and, because the procedure can be conducted aseptically, cells can be maintained in vitro after elutriation. A description of the technique, methods to determine flow rates and rotor speeds, and a preliminary separation of oyster hyalinocytes is presented PY 1988 SO American Fisheries Society Special Publication PU American Fisheries Society VL 18 BP 281 EP 285 ID 3118 ER EF