FN Archimer Export Format PT J TI Analysis of hemocyte parameters in Pacific oysters, Crassostrea gigas, reared in the field — Comparison of hatchery diploids and diploids from natural beds BT AF GAGNAIRE, Beatrice SOLETCHNIK, Patrick FAURY, Nicole KERDUDOU, Nolwenn LE MOINE, Olivier RENAULT, Tristan AS 1:1;2:2;3:2;4:1;5:2;6:1; FF 1:PDG-DOP-DCN-AGSAE-LGP;2:PDG-DOP-LER-LERPC;3:PDG-DOP-DCN-AGSAE-LGP;4:PDG-DOP-DCN-AGSAE-LGP;5:PDG-DOP-LER-LERPC;6:PDG-DOP-DCN-AGSAE-LGP; C1 IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France. IFREMER, Lab Environm & Ressources Pertuis Charentais, F-17390 La Tremblade, France. C2 IFREMER, FRANCE IFREMER, FRANCE SI LA TREMBLADE SE PDG-DOP-DCN-AGSAE-LGP PDG-DOP-LER-LERPC IN WOS Ifremer jusqu'en 2018 IF 1.735 TC 7 UR https://archimer.ifremer.fr/doc/2007/publication-3119.pdf LA English DT Article DE ;Hatchery;Natural beds;Diploids;Flow cytometry;Peroxidase activity;Esterase activity;Phagocytosis;Hemocytes;Crassostrea gigas;Pacific oyster AB Internal defence mechanisms of bivalves include the hemocytes which assume several functions. Relationships exist between environmental factors, physiological state of oysters, defence system and pathogens. Hatchery oysters are more and more produced and reared in the field. The characteristics and particularly the defence system of these animals are poorly documented. In this context, two field trials were carried out from May to September 2002 and from April to July 2003 in order to monitor hemocyte parameters of diploid oysters from natural beds and hatchery-produced diploid oysters using flow cytometry. Several hemocyte parameters (cell mortality, granulocyte percentage, phagocytosis, esterase and peroxidase activities) were monitored. For both oyster groups, trend of hemocyte parameters in 2002 and 2003 are reported. For several parameters, values tended to increase over the sampling period in both experiments. For both experiments, phagocytosis was higher for diploids from natural beds compared to hatchery diploids. This is the first study in which trend of hemocyte parameters from the Pacific oyster, Crassostrea gigas, was monitored with short sampling intervals and to compare hatchery diploids and diploids from natural beds. PY 2007 PD APR SO Aquaculture SN 0044-8486 PU Elsevier VL 264 IS 1-4 UT 000245659900048 BP 449 EP 456 DI 10.1016/j.aquaculture.2006.12.041 ID 3119 ER EF