FN Archimer Export Format PT J TI Screening of Vibrio isolates to develop an experimental infection model in the Pacific oyster Crassostrea gigas BT AF GAY, Melanie BERTHE, Franck LEROUX, Frederique AS 1:;2:;3:; FF 1:PDG-DRV-RA-LGP;2:PDG-DRV-RA-LGP;3:PDG-DRV-RA-LGP; C1 IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France. C2 IFREMER, FRANCE SI LA TREMBLADE LA TREMBLADE RONCE SE PDG-DRV-RA-LGP IN WOS Ifremer jusqu'en 2018 IF 1.583 TC 98 UR https://archimer.ifremer.fr/doc/2004/publication-3174.pdf LA English DT Article DE ;Crassostrea gias;Antagonism;Agonism;Virulence;Vibrio splendidus AB In an attempt to develop a reproducible experimental model of bacterial infection in Crassostrea gigas, oysters taken from very localised sub-populations suffering natural mortality outbreaks were used in cohabitation trials under laboratory conditions. From these trials, a collection of Vibrio strains was isolated from moribund and healthy oysters. In a second step, strains were experimentally tested for virulence by means of injection into healthy oysters. This screening revealed a span of virulence among isolated strains from none to medium. When pooling injected strains, results suggest increased virulence. Vibrio strains may have additive/synergistic action leading to higher C, gigas mortality rates in experimental challenges. Although the study initially aimed to develop a simple experimental model, a complex of interactions emerged between several bacterial strains during the pathogenic process in their molluscan host. Selected strains provide a suitable model of experimental disease for further studies and better understanding of bacterial interaction and pathogenesis in C. gigas. PY 2004 PD APR SO Diseases of aquatic organisms SN 0177-5103 PU Inter-Research VL 59 IS 1 UT 000222398000007 BP 49 EP 56 ID 3174 ER EF