FN Archimer Export Format
PT J
TI Image analysis : a new method for estimating triploidy in commercial bivalves
BT 
AF GERARD, Andre
   NACIRI, Yamama
   PEIGNON, Jean-Marie
   LEDU, Christophe
   PHELIPOT, Pascal
   NOIRET, Christophe
   PEUDENIER, Isabelle
   GRIZEL, Henri
AS 1:;2:;3:;4:;5:;6:;7:;8:;
FF 1:PDG-DP2S;2:;3:PDG-DOP-DCOP-LEADNC-LAC;4:PDG-DOP-DCN-AGSAE-LGP;5:PDG-DOP-DCN-AGSAE-LGP;6:;7:;8:PDG-DOP-DCM-SREU;
SI LA TREMBLADE
   SAINT VINCENT
   LA REUNION
SE PDG-DP2S
   PDG-DOP-DCOP-LEADNC-LAC
   PDG-DOP-DCN-AGSAE-LGP
   PDG-DOP-DCM-SREU
TC 0
UR https://archimer.ifremer.fr/doc/1994/publication-3200.pdf
LA English
DT Article
DE ;Estimation bivalve triploidy;Image analysis;Triploid;Genetic;Crassostrea gigas;Oysters
AB For determination of ploidy levels in bivalves,  three techniques are now routinely used: karyological determination. microfluorimetry and flow cytometry. This paper proposes an alternative technique which is based on determination of the optical density (OD) of stained nuclei using image analysis. A karyological and image analysis comparative experiment conducted on juveniles of Crassostrea gigas (Thunberg) and Ruditapes philippinarum (Adam & Reeve) is described and highlights the advantages of the image analysis technique. Initially developed on C. gigas and R. philippinarum, the image analysis method was easily extended to other bivalve species and to different developmental stages, as demonstrated by two experiments conducted on Ostrea edulis (L.). First, percentage of triploids was consistent from the D-larval to the adult stage of development, despite the use of different preparation techniques depending on the stage examined. Second, a rise in ploidy percentage demonstrated a differential mortality between diploid and triploid animals. Image analysis techniques arc cheaper than flow cytometry but more expensive than microfluorimetry. However, image analysis equipment can also be used for algae, eggs and spermatozoa counting,  and other purposes such as fluorescence measurements, analysis of autoradiographs and electropharesis gels, and measurement of immuno-histochemical reactions.
PY 1994
SO Aquaculture & Fisheries Management
VL 25
BP 697
EP 708
ID 3200
ER

EF