FN Archimer Export Format
PT J
TI Induced triploidy in the European clam, Ruditapes decussatus (L.) and performance of triploid larvae
BT 
AF GERARD, Andre
   NACIRI, Yamama
   NOIRET, Christophe
   LEDU, Christophe
   PEIGNON, Jean-Marie
AS 1:;2:;3:;4:;5:;
FF 1:PDG-DP2S;2:;3:;4:PDG-DOP-DCN-AGSAE-LGP;5:PDG-DOP-DCOP-LEADNC-LAC;
SI LA TREMBLADE
   SAINT VINCENT
SE PDG-DP2S
   PDG-DOP-DCN-AGSAE-LGP
   PDG-DOP-DCOP-LEADNC-LAC
TC 0
UR https://archimer.ifremer.fr/doc/1994/publication-3208.pdf
LA English
DT Article
DE ;Genetic;Ruditapes decussatus;Clam;Induced triploidy
AB For the first time, effective treatments using cytochalasin B were developed to induce triploidy in the European clam, Ruditapes decussatus (L.). The percentage of triploid embrios was assessed by karyological or image analysis. Two treatments (O.5 or 1 mg of cytochasin B (CB per ml of dimethylsulfoxide in 1 l of sea water) were applied at different times after fertilization (10 to 25 mn), for two different periods (15 and 20 min). Best results were obtained for a CB concentration of 1 mg/l. When treatment was applied 15 min after fertilization for a 20 min period, 94% and 95% of triploid embryos were obtained in two repeated experiments. At metamorphosis, the treated larvae appeared to be no smaller than the control larvae in all experiments. Thowever, in general, significantly higher mortalities for CB treatd batches weres found when compared with the untreated batch.
PY 1994
SO Aquaculture & Fisheries Management
VL 25
BP 769
EP 779
ID 3208
ER

EF