FN Archimer Export Format PT J TI A novel proteomic approach identifies new interaction partners for proliferating cell nuclear antigen BT AF MESIET CLADIERE, L NORAIS, Cédric KUHN, Joelle BRIFFOTAUX, Julien SLOOSTRA, Jerry W. FERRARI, Elena HUBSCHER, Ulrich FLAMENT, Didier MYLLYKALLIO, Hannu AS 1:2,3;2:2,3;3:2,3;4:1;5:4;6:5;7:5;8:1;9:2,3; FF 1:;2:;3:;4:PDG-DOP-DCB-EEP-LMEE;5:;6:;7:;8:PDG-DOP-DCB-EEP-LMEE;9:; C1 IFREMER, Ctr Brest, UMR 6197, Lab Microbiol Environm Extremes, F-29280 Plouzane, France. Univ Paris 11, CNRS, UMR 8621, F-91405 Orsay, France. Univ Paris 11, Inst Genet & Microbiol, F-91405 Orsay, France. Pepscan Syst, NL-8219 PH Lelystad, Netherlands. Univ Zurich, Inst Vet Biochem & Mol Biol, CH-8057 Zurich, Switzerland. C2 IFREMER, FRANCE UNIV PARIS 11, FRANCE UNIV PARIS 11, FRANCE PEPSCAN SYST, NETHERLANDS UNIV ZURICH, SWITZERLAND SI BREST SE PDG-DOP-DCB-EEP-LMEE IN WOS Ifremer jusqu'en 2018 copubli-france copubli-europe copubli-univ-france copubli-int-hors-europe IF 4.472 TC 37 UR https://archimer.ifremer.fr/doc/2007/publication-3297.pdf LA English DT Article DE ;Ribonuclease HII;Combinatorial peptide synthesis;PCNA AB During DNA replication and repair, many proteins bind to and dissociate in a highly specific and ordered manner from proliferating cell nuclear antigen (PCNA). We describe a combined approach of in silico searches at the genome level and combinatorial peptide synthesis to investigate the binding properties of hundreds of short PCNA-interacting peptides (PIP-peptides) to archaeal and eukaryal PCNAs. Biological relevance of our combined approach was demonstrated by identification an inactive complex of Pyrococcus abyssi ribonuclease HII with PCNA. Furthermore we show that PIP-peptides interact with PCNA largely in a sequence independent manner. Our experimental approach also identified many so far unidentified PCNA interacting peptides in a number of human proteins. (c) 2007 Elsevier Ltd. All rights reserved. PY 2007 PD OCT SO Journal of Molecular Biology SN 0022-2836 PU Elsevier VL 372 IS 5 UT 000249817500002 BP 1137 EP 1148 DI 10.1016/j.jmb.2007.06.056 ID 3297 ER EF