Determination of iron-porphyrin-like complexes at nanomolar levels in seawater

Type Article
Date 2007-04
Language English
Author(s) Vong Lilita1, Laes AgatheORCID2, Blain Stéphane1
Affiliation(s) 1 : Univ Aix Marseille 2, Ctr Oceanol Marseille, Lab Oceanog & Biogeochim, UMR CNRS 6535, F-13288 Marseille 09, France.
2 : IFREMER, Ctr Brest, Dept TSI, F-29280 Plouzane, France.
Source Analytica Chimica Acta (0003-2670) (Elsevier), 2007-04 , Vol. 588 , N. 2 , P. 237-244
DOI 10.1016/j.aca.2007.02.007
WOS© Times Cited 37
Keyword(s) Chemiluminescence, Complexation, Flow injection, Seawater, Porphyrin, Iron
Abstract A new method for the non-specific determination of iron-porphyrin-like complexes in natural waters has been developed. It is based on the chemiluminescent oxidation of the luminol in the presence of dioxygen (O-2) at pH 13. The method has been implemented in a FIA manifold that allowed the direct injection of seawater. The limit of detection is 0.11 nM of equivalent hemin (Fe-protoporphyrin IX). Fe2+, Fe3+, H2O2, siderophore (deferoxamin mesylate), humic acid and phytic acid did not interfere when the), were present at the concentrations expected in seawater. Metal free porphyrin and Mg, Cu, Co porphyrin complexes did not induce a significant chemiluminescent signal. Poisoned unfiltered samples could be stored for several weeks before analyses. The new method was successfully applied to the determination of the Fe-porphyrin complexes contained in cultured phytoplankton and in natural samples.
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