FN Archimer Export Format PT J TI Rotavirus VLP2/6: a new tool for tracking rotavirus in the marine environment BT AF LOISY, Fabienne ATMAR, Robert L. COHEN, Jean BOSCH, Albert LE GUYADER, Soizick AS 1:;2:;3:;4:;5:; FF 1:PDG-DEL-MP-MIC;2:;3:;4:;5:; C1 IFREMER, Microbiol Lab, F-44311 Nantes 3, France. Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA. CNRS, Unite Mixte, INRA, F-91198 Gif Sur Yvette, France. Univ Barcelona, Dept Microbiol, Enter Virus Grp, E-08028 Barcelona, Spain. C2 IFREMER, FRANCE BAYLOR COLL MED, USA CNRS, FRANCE UNIV BARCELONA, SPAIN SI NANTES SE PDG-DEL-MP-MIC IN WOS Ifremer jusqu'en 2018 copubli-france copubli-europe copubli-int-hors-europe IF 2.301 TC 10 UR https://archimer.ifremer.fr/doc/2004/publication-330.pdf LA English DT Article DE ;Stability;Tracer;Seawater;Virus like particles;Rotavirus AB The potential of rotavirus 2/6-virus-like-particles (VLP2/6) for use as tracers in the marine environment was investigated. The stability of bovine rotavirus (strain RF) and VLP2/6 in natural seawater at 25degreesC for six days was studied. ELISA and western blot methods were used to quantify the particles. The rates of decline of rotavirus particles and VLP2/6 were similar (approximately 0.5 log 10 per day). Western blot analysis showed that the integrity of capsid proteins VP2 and VP6 was conserved during the incubation time. These results demonstrate that VLP2/6 particles have the same stability in seawater as rotavirus particles. Thus, VLP2/6 can be used as a tracer, which should be of particular value for studying the fate of rotavirus particles in the marine environment. PY 2004 PD SEP SO Research in Microbiology SN 0923-2508 PU Elsevier VL 155 IS 7 UT 000223769300012 BP 575 EP 578 DI 10.1016/j.resmic.2004.04.002 ID 330 ER EF