FN Archimer Export Format
PT J
TI Single Nucleotide polymorphisms and their relationship to codon usage bias in the Pacific oyster Crassostrea gigas
OT Polymorphismes d'un nucléotide seul et leurs relations à l'usage d'un codon chez l'huître creuse du pacifique Crassostrea gigas
BT 
AF SAUVAGE, Christopher
   BIERNE, N
   LAPEGUE, Sylvie
   BOUDRY, Pierre
AS 1:1;2:2;3:1;4:1;
FF 1:PDG-DOP-DCN-AGSAE-LGP;2:;3:PDG-DOP-DCN-AGSAE-LGP;4:PDG-DOP-DCN-AGSAE-LGP;
C1 IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
   CNRS UMII, UMR 5554, Stn Mediterraneenne & Environm Littoral, Inst Sci Evolut Montpellier,Dept Biol Integrat, Sete, France.
C2 IFREMER, FRANCE
   CNRS UMII, FRANCE
SI LA TREMBLADE
SE PDG-DOP-DCN-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 2.871
TC 106
UR https://archimer.ifremer.fr/doc/2007/publication-3417.pdf
LA English
DT Article
DE ;Crassostrea gigas;Codon bias;Genetic diversity;SNP
AB DNA sequence polymorphism and codon usage bias were investigated in a set of 41 nuclear loci in the Pacific oyster Crassostrea gigas. Our results revealed a very high level of DNA polymorphism in oysters, in the order of magnitude of the highest levels reported in animals to date. A total of 290 single nucleotide polymorphisms (SNPs) were detected, 76 of which being localised in exons and 214 in non-coding regions. Average density of SNPs was estimated to be one SNP every 60 bp in coding regions and one every 40 bp in non-coding regions. Non-synonymous substitutions contributed substantially to the polymorphism observed in coding regions. The non-synonymous to silent diversity ratio was 0.16 on average, which is fairly higher to the ratio reported in other invertebrate species recognised to display large population sizes. Therefore, purifying selection does not appear to be as strong as it could have been expected for a species with a large effective population size. The level of non-synonymous diversity varied greatly from one gene to another, in accordance with varying selective constraints. We examined codon usage bias and its relationship with DNA polymorphism. The table of optimal codons was deduced from the analysis of an EST dataset, using EST counts as a rough assessment of gene expression. As recently observed in some other taxa, we found a strong and significant negative relationship between codon bias and non-synonymous diversity suggesting correlated selective constraints on synonymous and non-synonymous substitutions. Codon bias as measured by the frequency of optimal codons for expression might therefore provide a useful indicator of the level of constraint upon proteins in the oyster genome.  
PY 2007
PD DEC
SO Gene
SN 0378-1119
PU Elsevier
VL 406
IS 1-2
UT 000252500500004
BP 13
EP 22
DI 10.1016/j.gene.2007.05.011
ID 3417
ER

EF