FN Archimer Export Format
PT J
TI Evaluation of two library-independent microbial source tracking methods to identify sources of fecal contamination in french estuaries
BT 
AF GOURMELON, Michele
   CAPRAIS, Marie-Paule
   SEGURA, Raphael
   LE MENNEC, Cecile
   LOZACH, Solen
   PIRIOU, Jean-Yves
   RINCE, A
AS 1:1;2:1;3:1;4:1;5:1;6:2;7:3;
FF 1:PDG-DOP-DCN-EMP-MIC;2:PDG-DOP-DCN-EMP-MIC;3:PDG-DOP-DCN-EMP-MIC;4:PDG-DOP-DCN-EMP-MIC;5:PDG-DOP-DCN-EMP-MIC;6:PDG-DOP-LER-LERFBN;7:;
C1 IFREMER, Microbiol Lab, EMP, F-29280 Plouzane, France.
   IFREMER, LERCC, F-29280 Plouzane, France.
   Univ Caen, IRBA, INRA,Lab Microbiol Environm, USC 2017,EA 956, F-14032 Caen, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   UNIV CAEN, FRANCE
SI BREST
SE PDG-DOP-DCN-EMP-MIC
   PDG-DOP-LER-LERFBN
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 4.004
TC 104
UR https://archimer.ifremer.fr/doc/2007/publication-3535.pdf
LA English
DT Article
AB In order to identify the origin of the fecal contamination observed in French estuaries, two library-independent microbial source tracking (MST) methods were selected: (i) Bacteroidales host-specific 16S rRNA gene markers and (ii) F-specific RNA bacteriophage genotyping. The specificity of the Bacteroidales markers was evaluated on human and animal (bovine, pig, sheep, and bird) feces. Two human-specific markers (HF183 and HF134), one ruminant-specific marker (CF193'), and one pig-specific marker (PF163) showed a high level of specificity (>90%). However, the data suggest that the proposed ruminant-specific CF128 marker would be better described as an animal marker, as it was observed in all bovine and sheep feces and 96% of pig feces. F RNA bacteriophages were detected in only 21% of individual fecal samples tested, in 60% of pig slurries, but in all sewage samples. Most detected F RNA bacteriophages were from genotypes II and III in sewage samples and from genotypes I and IV in bovine, pig, and bird feces and from pig slurries. Both MST methods were applied to 28 water samples collected from three watersheds at different times. Classification of water samples as subject to human, animal, or mixed fecal contamination was more frequent when using Bacteroidales markers (82.1% of water samples) than by bacteriophage genotyping (50%). The ability to classify a water sample increased with increasing Escherichia coli or enterococcus concentration. For the samples that could be classified by bacteriophage genotyping, 78% agreed with the classification obtained from Bacteroidales markers.
PY 2007
PD MAY
SO Applied and environmental microbiology
SN 0099-2240
PU American society for microbiology
VL 73
IS 15
UT 000248587600019
BP 4857
EP 4866
DI 10.1128/AEM.03003-06
ID 3535
ER

EF