Analysis of the first genome of a hyperthermophilic marine virus-like particle, PAV1, isolated from Pyrococcus abyssi

Type Article
Date 2007-04
Language English
Author(s) Geslin Claire3, Gaillard Melusine2, Flament DidierORCID1, Rouault Karen1, 2, Le Romancer Marc4, Prieur Daniel4, Erauso Gael3
Affiliation(s) 1 : IFREMER, CNRS, Lab Microbiol Environm Extremes, UMR 6197,UBO, F-29280 Plouzane, France.
2 : INSERM, Lab Genet Mol & Genet Epidemiol, U613, F-29220 Brest, France.
Source Journal of bacteriology (0021-9193) (American society for microbiology), 2007-04 , Vol. 189 , N. 12 , P. 4510-4519
DOI 10.1128/JB.01896-06
WOS© Times Cited 53
Abstract Only one virus-like particle (VLP) has been reported from hyperthermophilic Euryarchaeotes. This VLP, named PAV1 is shaped like a lemon and was isolated from a strain of "Pyrococcus abyssi," a deep-sea isolate. Its genome consists of a double-stranded circular DNA of 18 kb which is also present at a high copy number (60 per chromosome) free within the host cytoplasm but is not integrated into the host chromosome. Here, we report the results of complete analysis of the PAV1 genome. All the 25 predicted genes, except 3, are located on one DNA strand. A transcription map has been made by using a reverse transcription-PCR assay. All the identified open reading frames (ORFs) are transcribed. The most significant similarities relate to four ORFs. ORF 180a shows 31% identity with ORF 181 of the pRT1 plasmid isolated from Pyrococcus sp. strain JT1. ORFs 676 and 678 present similarities with a concanavalin A-like lectin/glucanase domain, which could be involved in the process of host-virus recognition, and ORF 59 presents similarities with the transcriptional regulator CopG. The genome of PAV1displays unique features at the nucleic and proteinic level, indicating that PAV1 should be attached at least to a novel genus or virus family.
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Geslin Claire, Gaillard Melusine, Flament Didier, Rouault Karen, Le Romancer Marc, Prieur Daniel, Erauso Gael (2007). Analysis of the first genome of a hyperthermophilic marine virus-like particle, PAV1, isolated from Pyrococcus abyssi. Journal of bacteriology, 189(12), 4510-4519. Publisher's official version : , Open Access version :