FN Archimer Export Format
PT J
TI Analysis of the first genome of a hyperthermophilic marine virus-like particle, PAV1, isolated from Pyrococcus abyssi
BT 
AF GESLIN, Claire
   GAILLARD, Melusine
   FLAMENT, Didier
   ROUAULT, Karen
   LE ROMANCER, Marc
   PRIEUR, Daniel
   ERAUSO, Gael
AS 1:3;2:2;3:1;4:1,2;5:4;6:4;7:3;
FF 1:;2:;3:PDG-DOP-DCB-EEP-LMEE;4:PDG-DOP-DCB-EEP-LMEE;5:;6:;7:;
C1 IFREMER, CNRS, Lab Microbiol Environm Extremes, UMR 6197,UBO, F-29280 Plouzane, France.
   INSERM, Lab Genet Mol & Genet Epidemiol, U613, F-29220 Brest, France.
C2 IFREMER, FRANCE
   INSERM, FRANCE
   UBO, FRANCE
   IUEM, FRANCE
SI BREST
SE PDG-DOP-DCB-EEP-LMEE
   IUEM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 4.013
TC 53
UR https://archimer.ifremer.fr/doc/2007/publication-3536.pdf
LA English
DT Article
AB Only one virus-like particle (VLP) has been reported from hyperthermophilic Euryarchaeotes. This VLP, named PAV1 is shaped like a lemon and was isolated from a strain of "Pyrococcus abyssi," a deep-sea isolate. Its genome consists of a double-stranded circular DNA of 18 kb which is also present at a high copy number (60 per chromosome) free within the host cytoplasm but is not integrated into the host chromosome. Here, we report the results of complete analysis of the PAV1 genome. All the 25 predicted genes, except 3, are located on one DNA strand. A transcription map has been made by using a reverse transcription-PCR assay. All the identified open reading frames (ORFs) are transcribed. The most significant similarities relate to four ORFs. ORF 180a shows 31% identity with ORF 181 of the pRT1 plasmid isolated from Pyrococcus sp. strain JT1. ORFs 676 and 678 present similarities with a concanavalin A-like lectin/glucanase domain, which could be involved in the process of host-virus recognition, and ORF 59 presents similarities with the transcriptional regulator CopG. The genome of PAV1displays unique features at the nucleic and proteinic level, indicating that PAV1 should be attached at least to a novel genus or virus family.
PY 2007
PD APR
SO Journal of bacteriology
SN 0021-9193
PU American society for microbiology
VL 189
IS 12
UT 000247231700021
BP 4510
EP 4519
DI 10.1128/JB.01896-06
ID 3536
ER

EF