TY - JOUR T1 - Characterization of actin genes in Bonamia ostreae and their application to phylogeny of the Haplosporidia A1 - Lopez Flores,L A1 - Suarez Santiago,V A1 - Longet,D A1 - Saulnier,Denis A1 - Chollet,Bruno A1 - Arzul,Isabelle AD - IFREMER, Lab Genet & Pathol, Stn Tremblade, F-17390 La Tremblade, France. AD - Univ Granada, Fac Sci, Dept Bot, E-18071 Granada, Spain. AD - Univ Geneva, Dept Zool & Anim Biol, CH-1211 Geneva, Switzerland. UR - https://archimer.ifremer.fr/doc/00000/3562/ DO - 10.1017/S0031182007003307 KW - Molecular phylogeny KW - Actin gene KW - Ostrea edulis parasite KW - Bonamia ostreae N2 - Bonamia ostreae is a protozoan parasite that infects the European flat oyster Ostrea edulis, causing systemic infections and resulting in massive mortalities in populations of this valuable bivalve species. In this work, we have characterized B. ostreae actin genes and used their sequences for a phylogenetic analysis. Design of different primer sets was necessary to amplify the central coding region of actin genes of B. ostreae. Characterization of the sequences and their amplification in different samples demonstrated the presence of 2 intragenomic actin genes in B. ostreae, without any intron. The phylogenetic analysis placed B. ostreae in a clade with Minchinia tapetis, Minchinia teredinis and Haplosporidium costale as its closest relatives, and demonstrated that the paralogous actin genes found in Bonamia resulted from a duplication of the original actin gene after the Bonamia origin. Y1 - 2007/12 PB - Cambridge University Press JF - Parasitology SN - 0031-1820 VL - 134 IS - 14 SP - 1941 EP - 1948 ID - 3562 ER -