FN Archimer Export Format PT J TI Characterization of actin genes in Bonamia ostreae and their application to phylogeny of the Haplosporidia BT AF LOPEZ FLORES, L SUAREZ SANTIAGO, V LONGET, D SAULNIER, Denis CHOLLET, Bruno ARZUL, Isabelle AS 1:1;2:2;3:3;4:1;5:1;6:1; FF 1:;2:;3:;4:PDG-DOP-DCN-AGSAE-LGP;5:PDG-DOP-DCN-AGSAE-LGP;6:PDG-DOP-DCN-AGSAE-LGP; C1 IFREMER, Lab Genet & Pathol, Stn Tremblade, F-17390 La Tremblade, France. Univ Granada, Fac Sci, Dept Bot, E-18071 Granada, Spain. Univ Geneva, Dept Zool & Anim Biol, CH-1211 Geneva, Switzerland. C2 IFREMER, FRANCE UNIV GRANADA, SPAIN UNIV GENEVA, SWITZERLAND SI LA TREMBLADE SE PDG-DOP-DCN-AGSAE-LGP IN WOS Ifremer jusqu'en 2018 copubli-europe copubli-int-hors-europe IF 2.081 TC 17 UR https://archimer.ifremer.fr/doc/2007/publication-3562.pdf LA English DT Article DE ;Molecular phylogeny;Actin gene;Ostrea edulis parasite;Bonamia ostreae AB Bonamia ostreae is a protozoan parasite that infects the European flat oyster Ostrea edulis, causing systemic infections and resulting in massive mortalities in populations of this valuable bivalve species. In this work, we have characterized B. ostreae actin genes and used their sequences for a phylogenetic analysis. Design of different primer sets was necessary to amplify the central coding region of actin genes of B. ostreae. Characterization of the sequences and their amplification in different samples demonstrated the presence of 2 intragenomic actin genes in B. ostreae, without any intron. The phylogenetic analysis placed B. ostreae in a clade with Minchinia tapetis, Minchinia teredinis and Haplosporidium costale as its closest relatives, and demonstrated that the paralogous actin genes found in Bonamia resulted from a duplication of the original actin gene after the Bonamia origin. PY 2007 PD DEC SO Parasitology SN 0031-1820 PU Cambridge University Press VL 134 IS 14 UT 000251699200003 BP 1941 EP 1948 DI 10.1017/S0031182007003307 ID 3562 ER EF