FN Archimer Export Format PT J TI Quantitative relationship of two viruses (MrNV and XSV) in white-tail disease of Macrobrachium rosenbergii BT AF ZHANG, HJ WANG, HM YUAN, JF LI, LJ ZHANG, JH BONAMI, Jean-Robert SHI, ZL AS 1:1;2:1;3:1;4:1;5:1;6:2,3;7:1; FF 1:;2:;3:;4:;5:;6:;7:; C1 Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China. Univ Montpellier 2, CNRS, ECOLAG, UMR5119, Montpellier, France. C2 CHINESE ACAD SCI, CHINA UNIV MONTPELLIER, FRANCE IFREMER, FRANCE SI MONTPELLIER SE PDG-DOP-DCN-AGSAE-GPIA IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france copubli-int-hors-europe copubli-sud IF 1.509 TC 41 UR https://archimer.ifremer.fr/doc/2006/publication-3568.pdf LA English DT Article DE ;White tail disease;Real time RT PCR;Extra small virus;Nodavirus;Macrobrachium rosenbergii AB Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) were purified from diseased freshwater prawns M rosenbergii and used to infect healthy post-larvae (PL) by an immersion method. Three groups of prawns were challenged with various combined doses of MrNV and XSV. Signs of white-tail disease (WTD) were observed in Groups 1 and 2, which had been challenged with combinations containing relatively high proportions of MrNV and low proportions of XSV. By contrast there was little sign of WTD in Group 3, which had been challenged with a higher proportion of XSV than MrNV. A 2-step Taqman real-time RT-PCR was developed and applied to quantify viral copy numbers in each challenged PL. Results showed that genomic copies of both viruses were much higher in Groups 1 and 2 than they were in Group 3, indicating that MrNV plays a key role in WTD of M rosenbergii. The linear correlation between MrNV and XSV genome copies in infected prawns demonstrated that XSV is a satellite virus, dependent on MrNV, but its role in pathogenicity of WTD remains unclear. PY 2006 PD JUN SO Diseases of aquatic organisms SN 0177-5103 PU Inter-Research VL 71 IS 1 UT 000240510300002 BP 11 EP 17 DI 10.3354/dao071011 ID 3568 ER EF