FN Archimer Export Format
PT J
TI Characterisation of physiological and immunological differences between Pacific oysters (Crassostrea gigas) genetically selected for high or low survival to summer mortalities and fed different rations under controlled conditions
BT 
AF DELAPORTE, Maryse
   SOUDANT, P
   LAMBERT, C
   JEGADEN, M
   MOAL, Jeanne
   POUVREAU, Stephane
   DEGREMONT, Lionel
   BOUDRY, Pierre
   SAMAIN, Jean-Francois
AS 1:2;2:1;3:1;4:1;5:2;6:2;7:3;8:3;9:2;
FF 1:PDG-DOP-DCB-PFOM-PI;2:;3:;4:;5:PDG-DOP-DCB-PFOM-PI;6:PDG-DOP-DCB-PFOM-PI;7:PDG-DOP-DCN-AGSAE-LGP;8:PDG-DOP-DCN-AGSAE-LGP;9:PDG-DOP-DCB-PFOM-PI;
C1 Univ Bretagne Occidentale, Inst Univ Europeen Mer, UMR 6539, Lab Sci Environm Marin, F-29280 Plouzane, France.
   IFREMER, Ctr Brest, Lab Physiol Invertebres, F-29280 Plouzane, France.
   IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
C2 UBO, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
SI BREST
   ARGENTON
   LA TREMBLADE
SE PDG-DOP-DCB-PFOM-PI
   PDG-DOP-DCN-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 1.75
TC 35
UR https://archimer.ifremer.fr/doc/2007/publication-3578.pdf
LA English
DT Article
DE ;Summer mortality;Reproduction;Reactive oxygen species ROS;Hemocyte parameters;Genetic selection;Crassostrea gigas
AB Within the framework of a national scientific program named "MORtalites ESTivales de l'huitre creuse Crassostrea gigas" (MOREST), a family-based experiment was developed to study the genetic basis of resistance to summer mortality in the Pacific oyster, Crassostrea gigas. As part of the MOREST project, the second generation of three resistant families and two susceptible families were chosen and pooled into two respective groups: "R" and "S". These two groups of oysters were conditioned for 6 months on two food levels (4% and 12% of oyster soft-tissue dry weight in algal dry weight per day) with a temperature gradient that mimicked the Marennes-Oleron natural cycle during the oyster reproductive period. Oyster mortality remained low for the first two months, but then rapidly increased in July when seawater temperature reached 19 degrees C and above. Mortality was higher in "S" oysters than in "R" oysters, and also higher in oysters fed the 12% diet than those fed 4%, resulting in a decreasing, relative order in cumulative mortality as follows; 12% "S" > 12% "R" >4% "S" >4% "R". Although the observed mortality rates were lower than those previously observed in the field, the mortality differential between "R" and "S" oysters was similar. Gonadal development, estimated by tissue lipid content, followed a relative order yielding a direct, positive relationship between reproductive effort and mortality as we reported precedently by quantitative histology. Regarding hemocyte parameters, one of the most striking observations was that reactive oxygen species (ROS) production was significantly higher in "S" oysters than in "R" oysters in May and June, regardless of food level. The absence of known environmental stress under these experimental conditions suggests that the ROS increase in "S" oyster could be related to their higher reproductive activity. Finally, a higher increase in hyalinocyte counts was observed for "S" oysters, compared to "R" oysters, in July, just before mortality. Taken together, our results suggest an association of genetically based resistance to summer mortality, reproductive strategy and hemocyte parameters. (C) 2007 Elsevier B.V. All rights reserved.
PY 2007
PD DEC
SO Journal of Experimental Marine Biology and Ecology
SN 0022-0981
PU Elsevier
VL 353
IS 1
UT 000252014900005
BP 45
EP 57
DI 10.1016/j.jembe.2007.09.003
ID 3578
ER

EF