FN Archimer Export Format
PT J
TI Anti-rabbit immunoglobulin G detection in complex medium by PM-RAIRS and QCM Influence of the antibody immobilisation method
BT 
AF BRIAND, Elisabeth
   SALMAIN, M
   COMPERE, Chantal
   PRADIER, C
AS 1:1;2:2;3:3;4:1;
FF 1:;2:;3:PDG-DOP-DCB-ERT-IC;4:;
C1 Univ Paris 06, CNRS, UMR 7609, Lab React Surface, F-75252 Paris 05, France.
   Ecole Natl Super Chim Paris, UMR 7576, Lab Chim & Biochim Complexes Mol, F-75231 Paris, France.
   IFREMER, Ctr Brest, Dept Essais & Rech Technol Interfaces & Capteurs, F-29280 Plouzane, France.
C2 UNIV PARIS 06, FRANCE
   ENSCP, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-DOP-DCB-ERT-MS
   PDG-DOP-DCB-ERT-IC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 5.061
TC 39
UR https://archimer.ifremer.fr/doc/2007/publication-3580.pdf
LA English
DT Article
DE ;RIgG immobilisation;PM RAIRS;QCM;Immunosensors
AB Two antibody immobilisation procedures were compared to set up an immunosensor for goat anti-rabbit immunoglobulin (anti-rIgG), i.e. rIgG covalently bound or immobilised via affinity to protein A (PrA). In both cases, the first layer of protein was covalently bound to a mixed self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) and mercaptohexanol (C6OH) on a gold surface. The elaboration of the sensitive surfaces, as well as their selectivity and sensitivity were studied step by step by polarization modulation-reflection absorption infra-red spectroscopy (PM-RAIRS) and quartz crystal trticrobalance (QCM) with impedance measurement. QCM measurements showed that the viscoelastic properties of the antibody layer were markedly modified during the antigen recognition when the antibody was bound by affinity to PrA. The specific detection of antigen within a complex medium was assessed by PM-RAIRS thanks to the grafting of cobalt-carbonyl probes. Affinity constants between the immobilised rIgG and the anti-rIgG were determined from PM-RAIRS analysis. (c) 2006 Elsevier B.V. All rights reserved.
PY 2007
PD JUL
SO Biosensors and Bioelectronics
SN 0956-5663
PU Elsevier
VL 22
IS 12
UT 000247555300017
BP 2884
EP 2890
DI 10.1016/j.bios.2006.12.009
ID 3580
ER

EF