FN Archimer Export Format PT J TI Molecular characterization of penaeidins from two Atlantic Brazilian shrimp species, Farfantepenaeus paulensis and Litopenaeus schmitti BT AF BARRACCO, Margherita DE LORGERIL, Julien GUEGUEN, Yannick BACHERE, Evelyne AS 1:1,2;2:1;3:1;4:1; FF 1:PDG-DOP-DCN-AGSAE-GPIA;2:PDG-DOP-DCN-AGSAE-GPIA;3:PDG-DOP-DCN-AGSAE-GPIA;4:PDG-DOP-DCN-AGSAE-GPIA; C1 Univ Montpellier 2, IFREMER, CNRS, UMR Genome Populat Interact Adaptat 5171, F-34095 Montpellier, France. Univ Fed Santa Catarina, Dept Biol Celular Embriol & Genet, Florianopolis, SC, Brazil. C2 IFREMER, FRANCE UNIV FED SANTA CATARINA, BRAZIL SI MONTPELLIER SE PDG-DOP-DCN-AGSAE-GPIA IN WOS Ifremer jusqu'en 2018 copubli-int-hors-europe copubli-sud IF 2.057 TC 15 UR https://archimer.ifremer.fr/doc/2005/publication-3582.pdf LA English DT Article DE ;Immuno detection;Molecular cloning;Litopenaeus schmitti;Farfantepenaeus paulensis;Brazilian penaeid shrimps;Hemocytes;Antimicrobial peptides;Penaeidins AB We report here the molecular cloning of new members of the penaeidin family from two Atlantic penaeids from Brazil, Litopenaeus schmitti and Farfantepenaeus paulensis. The presence of penaeidins in the granular hemocytes of both shrimps was first evidenced by immunofluorescence, using polyclonal antibodies raised against L. vannamei penaeidin Litvan PEN3-1. cDNAs from the hemocytes of both Brazilian species were obtained by reverse transcription and the sequences encoding penaeidins were amplified by PCR, using primers based on penaeidin consensus sequences. Five penaeidin clones were obtained. According to the international penaeidin classification (PenBase, http://www.penbase.immunaqua.com), the deduced amino acid sequences of two clones from L. schmitti and two from F. paulensis belong to the PEN2 subgroup and one clone from L. schmitti to the PEN4 subgroup of penaeidins. Surprisingly, no penacidin from the PEN3 subgroup was obtained in both shrimp species, even though this subgroup appears to be the most commonly expressed in the hemocytes of penaeids. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. PY 2005 PD SEP SO FEMS Microbiology Letters SN 0378-1097 PU Blackwell science VL 250 IS 1 UT 000231403200016 BP 117 EP 120 DI 10.1016/j.femsle.2005.06.055 ID 3582 ER EF