FN Archimer Export Format
PT J
TI Pathogenicity of Vibrio penaeicida or white shrimp Litopenaeus vannamei: a cysteine protease-like exotoxin as a virulence factor
BT 
AF AGUIRRE GUZMAN, G
   ASCENCIO, F
   SAULNIER, Denis
AS 1:1;2:1;3:2;
FF 1:;2:;3:PDG-DOP-DCN-AGSAE-LGP;
C1 Ctr Invest Biol Noroeste, Unidad Patol Marina, La Paz, Baja California, Mexico.
   IFREMER, French Res Inst Exploitat Sea, Lab Genet & Pathol, F-17390 La Tremblade, France.
C2 CIBNOR, MEXICO
   IFREMER, FRANCE
SI LA TREMBLADE
SE PDG-DOP-DCN-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
   copubli-sud
IF 1.361
TC 10
UR https://archimer.ifremer.fr/doc/2005/publication-3618.pdf
LA English
DT Article
DE ;Pathogenicity;Exotoxin;Shrimp;Litopenaeus vannamei;Cysteine protease;Vibrio penaeicida
AB The pathogenicity of Vibrio penaeicida Strains KH-1 and AM101, their culture-free supernatant (CFS), and their protein fraction obtained by 40% of ammonium sulfate precipitation (PFs40) were assessed in experimental challenges against juvenile Litopenaeus vannamei. Live Vibrio cells, CFS, and PFs40 from the AM101 strain produced a significantly higher mortality (p < 0.05) compared to the KH-1 strain. Toxicity and median lethal doses (LD50) of Fast Protein Liquid Chromatography (FPLC) products were evaluated on L. vannamei. The first FPLC fraction sample (A) from PFs40 of the AM101 strain displayed LD50 values of 1.68 and 5.61 mu g protein ind.(-1), respectively. The second FPLC process from Fraction A showed a peak (A1) also with toxic effects to shrimp. PFs40, Fraction A, and Peak At showed a 38.5 kDa molecular band (SDS-PAGE), with activity on a gelatin protease zymogram. The lethal effect of PFs40 and Fraction A was inhibited by Proteinase K, CuCl2, E-64, and heat (60 and 100 degrees C) treatments, but was not inhibited by EDTA-Na-2, aprotinin, and soy trypsin treatments. These results and the zymogram inhibition test suggest the presence of a cysteine protease-like proteinaceous exotoxin as a dominant protease, secreted by V. penaeicida Strain AM101.
PY 2005
PD NOV
SO Diseases of aquatic organisms
SN 0177-5103
PU Inter-Research
VL 67
IS 3
UT 000235022400003
BP 201
EP 207
DI 10.3354/dao067201
ID 3618
ER

EF