FN Archimer Export Format
PT J
TI Regulation of the somatotropic axis by dietary factors in rainbow trout (Oncorhynchus mykiss)
BT 
AF GOMEZ REQUENI, P
   CALDUCH GINER, J
   DE CELIS, S
   MEDALE, Francoise
   KAUSHIK, Sadasivam
   PEREZ SANCHEZ, Jaume
AS 1:2;2:2;3:2;4:1,3;5:1,3;6:3;
FF 1:;2:;3:;4:;5:;6:;
C1 IFREMER, Unite Mixte INRA, Lab Nutr Poissons, F-64310 St Pee Sur Nivelle, France.
   CSIC, Inst Acuicultura Torre Sal, Castellon, Spain.
C2 IFREMER, FRANCE
   CSIC, SPAIN
   INRA, FRANCE
SI ST PEE SUR NIVELLE
SE PDG-DOP-DCB-PFOM-ARN
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-europe
IF 2.967
TC 45
UR https://archimer.ifremer.fr/doc/2005/publication-3620.pdf
LA English
DT Article
DE ;Insulin like growth factor;Growth hormone receptor;Growth hormone;Plant proteins;Rainbow trout
AB The activity of the somatotropic axis was analysed in juvenile rainbow trout (Oncorhynchus mykiss) fed either a fishmeal-based diet (FM) or graded levels of plant proteins to replace 50 % (PP50 diet), 75 % (PP75 diet) or 100 % (PP100 diet) of the fishmeal protein. For this purpose, partial cloning and sequencing of the gene encoding rainbow trout growth hormone receptor (GHR) was first accomplished by RT-PCR, using degenerate primers based on the sequences of non-salmonid fish GHR. Growth rates and energy retention were lowered by the PP75 and PP100 diets and a concurrent and progressive increase in plasma levels of growth hormone (GH) was found. However, no changes in hepatic GH binding and total plasma insulin-like growth factor (IGF)-I levels were observed among the four experimental groups. This fact agrees with the lack of changes in hepatic measurements of GHR and IGF-I transcripts. No consistent changes in IGF transcripts were found in peri-visceral adipose tissue and skeletal muscle, but GHR mRNA was up-regulated in the peri-visceral adipose tissue of fish fed the PP75 and PP100 diets, which would favour the lipolytic action of GH. Two specific bands (47 and 33 kDa) of IGF-binding proteins were found in the plasma of all analysed fish, but the sum of the two integrated areas increased progressively with plant protein supply, which might reflect a reduced free IGF availability. Therefore, in our experimental model, the growth impairment could be due, at least in part, to a lowered availability of biologically active IGF (free IGF fraction) rather than to liver GH desensitization or defect in IGF synthesis and release at the systemic and/or paracrine-autocrine level.
PY 2005
PD SEP
SO British Journal of Nutrition
SN 0007-1145
PU Cambridge University Press
VL 94
IS 3
UT 000231978600008
BP 353
EP 361
DI 10.1079/BJN20051521
ID 3620
ER

EF