TY - JOUR T1 - Continuous enrichment culture and molecular monitoring to investigate the microbial diversity of thermophiles inhabiting deep-sea hydrothermal ecosystems A1 - Postec,Anne A1 - Urios,Laurent A1 - Lesongeur,Francoise A1 - Ollivier,Bernard A1 - Querellou,Joel A1 - Godfroy,Anne AD - IFREMER, Ctr Brest, Lab Microbiol Environm Extremes, UMR 6197, F-29280 Plouzane, France. AD - Univ Provence & Mediterranee, BAIM, IFR,Lab Microbiol, UR 101,Inst Rech Pour Dev, F-13288 Marseille, France. UR - https://archimer.ifremer.fr/doc/00000/364/ DO - 10.1007/s00284-004-4443-z KW - Sequence alignment KW - Anaerobic digester KW - Thermotogales KW - Thermophile KW - Deep sea vent N2 - The microflora developing during a continuous enrichment culture from a hydrothermal chimney sample was investigated by molecular methods. The culture was performed in a gas-lift bioreactor under anaerobic conditions, at 90 degrees C and pH 6.5, on a complex medium containing sulfur as the terminal electron acceptor. Archaeal and bacterial diversity was studied. Microorganisms affiliated with the genera Pyrococcus, Marinitoga, and Bacillus were detected through DGGE analysis of 16S rDNA. Additional sequences phylogenetically related to Thermococus and epsilon-Proteobacteria were detected by cloning and sequencing of 16S rDNA from two samples of the enrichment culture. In comparison, the sequences retrieved from cloning analysis from an enrichment culture performed in a flask (batch condition) using the same Culture medium showed that only members of the genus Thermococcus were cultivated. Therefore, continuous enrichment culture using the gas-lift bioreactor can be considered as an efficient and improved method for investigating microbial communities originating from deep-sea hydrothermal vents. Y1 - 2005/03 PB - Springer JF - Current Microbiology SN - 0343-8651 VL - 50 IS - 3 SP - 138 EP - 144 ID - 364 ER -