FN Archimer Export Format
PT J
TI Continuous enrichment culture and molecular monitoring to investigate the microbial diversity of thermophiles inhabiting deep-sea hydrothermal ecosystems
BT 
AF POSTEC, Anne
   URIOS, Laurent
   LESONGEUR, Francoise
   OLLIVIER, Bernard
   QUERELLOU, Joel
   GODFROY, Anne
AS 1:;2:;3:;4:;5:;6:;
FF 1:PDG-DOP-DCB-EEP-LMEE;2:PDG-DOP-DCB-EEP-LMEE;3:PDG-DOP-DCB-EEP-LMEE;4:;5:PDG-DOP-DCB-EEP-LMEE;6:PDG-DOP-DCB-EEP-LMEE;
C1 IFREMER, Ctr Brest, Lab Microbiol Environm Extremes, UMR 6197, F-29280 Plouzane, France.
   Univ Provence & Mediterranee, BAIM, IFR,Lab Microbiol, UR 101,Inst Rech Pour Dev, F-13288 Marseille, France.
C2 IFREMER, FRANCE
   UNIV PROVENCE & MEDITERRANEE, FRANCE
SI BREST
SE PDG-DOP-DCB-EEP-LMEE
IN WOS Ifremer jusqu'en 2018
   copubli-univ-france
IF 1.059
TC 20
TU Centre national de la recherche scientifique
   Institut français de recherche pour l'exploitation de la mer
   Université de Bretagne Occidentale
UR https://archimer.ifremer.fr/doc/2005/publication-364.pdf
LA English
DT Article
CR ATOS
BO Unknown
DE ;Sequence alignment;Anaerobic digester;Thermotogales;Thermophile;Deep sea vent
AB The microflora developing during a continuous enrichment culture from a hydrothermal chimney sample was investigated by molecular methods. The culture was performed in a gas-lift bioreactor under anaerobic conditions, at 90 degrees C and pH 6.5, on a complex medium containing sulfur as the terminal electron acceptor. Archaeal and bacterial diversity was studied. Microorganisms affiliated with the genera Pyrococcus, Marinitoga, and Bacillus were detected through DGGE analysis of 16S rDNA. Additional sequences phylogenetically related to Thermococus and epsilon-Proteobacteria were detected by cloning and sequencing of 16S rDNA from two samples of the enrichment culture. In comparison, the sequences retrieved from cloning analysis from an enrichment culture performed in a flask (batch condition) using the same Culture medium showed that only members of the genus Thermococcus were cultivated. Therefore, continuous enrichment culture using the gas-lift bioreactor can be considered as an efficient and improved method for investigating microbial communities originating from deep-sea hydrothermal vents.
PY 2005
PD MAR
SO Current Microbiology
SN 0343-8651
PU Springer
VL 50
IS 3
UT 000228643300003
BP 138
EP 144
DI 10.1007/s00284-004-4443-z
ID 364
ER

EF