Optimisation of growth conditions for continuous culture of the hyperthermophilic archaeon Thermococcus hydrothermalis and development of sulphur-free defined and minimal media

Type Article
Date 2005
Language English
Author(s) Postec Anne1, Pignet Patricia1, Cueff-Gauchard Valerie1, Schmitt Anne1, Querellou Joel1, Godfroy AnneORCID1
Affiliation(s) 1 : Ifremer, Ctr Brest, UMR 6197 , Lab Microbiol Environm Extremes, F-29280 Plouzane, France.
Source Research in Microbiology (0923-2508) (Elsevier), 2005 , Vol. 156 , N. 1 , P. 82-87
DOI 10.1016/j.resmic.2004.08.001
WOS© Times Cited 12
Keyword(s) Bioreactor, Defined and minimal media, Culture optimisation, Gas lift bioreactor, Thermococcus hydrothermalis
Abstract The hyperthermophilic archaeon Thermococcus hydrothermalis was cultivated in continuous culture in a gas-lift bioreactor in the absence of elemental sulphur on both proteinaceous and maltose-containing media. Optimal conditions (pH, temperature and gas flow rate), determined on complex media that yielded maximal growth rate and maximal steady state cell density, were obtained at 80degreesC, pH 6 and gas sparging at 0.2 v v(-1) min(-1). Higher steady state cell densities were obtained on a medium containing maltose and yeast extract. In order to design a defined and minimal media, the nutritional requirements of T hydrothermalis were then investigated using continuous culture in the absence of elemental sulphur in the gas-lift bioreactor. First, the complex nutriments were replaced and a defined medium containing maltose, 19 amino acids and the two nitrogenous bases adenine and thymine, was determined. Secondly, selective feedings and withdrawal of amino acids showed requirements for 14 amino acids.
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Postec Anne, Pignet Patricia, Cueff-Gauchard Valerie, Schmitt Anne, Querellou Joel, Godfroy Anne (2005). Optimisation of growth conditions for continuous culture of the hyperthermophilic archaeon Thermococcus hydrothermalis and development of sulphur-free defined and minimal media. Research in Microbiology, 156(1), 82-87. Publisher's official version : https://doi.org/10.1016/j.resmic.2004.08.001 , Open Access version : https://archimer.ifremer.fr/doc/00000/365/