FN Archimer Export Format
PT J
TI Optimisation of growth conditions for continuous culture of the hyperthermophilic archaeon Thermococcus hydrothermalis and development of sulphur-free defined and minimal media
BT 
AF POSTEC, Anne
   PIGNET, Patricia
   CUEFF-GAUCHARD, Valerie
   SCHMITT, Anne
   QUERELLOU, Joel
   GODFROY, Anne
AS 1:1;2:1;3:1;4:1;5:1;6:1;
FF 1:PDG-DOP-DCB-EEP-LMEE;2:PDG-DOP-DCB-EEP-LMEE;3:PDG-DOP-DCB-EEP-LMEE;4:PDG-DOP-LER-LERMPL;5:PDG-DOP-DCB-EEP-LMEE;6:PDG-DOP-DCB-EEP-LMEE;
C1 Ifremer, Ctr Brest, UMR 6197 , Lab Microbiol Environm Extremes, F-29280 Plouzane, France.
C2 IFREMER, FRANCE
SI BREST
   LA TRINITE
SE PDG-DOP-DCB-EEP-LMEE
   PDG-DOP-LER-LERMPL
IN WOS Ifremer jusqu'en 2018
IF 2.426
TC 12
UR https://archimer.ifremer.fr/doc/2005/publication-365.pdf
LA English
DT Article
DE ;Bioreactor;Defined and minimal media;Culture optimisation;Gas lift bioreactor;Thermococcus hydrothermalis
AB The hyperthermophilic archaeon Thermococcus hydrothermalis was cultivated in continuous culture in a gas-lift bioreactor in the absence of elemental sulphur on both proteinaceous and maltose-containing media. Optimal conditions (pH, temperature and gas flow rate), determined on complex media that yielded maximal growth rate and maximal steady state cell density, were obtained at 80degreesC, pH 6 and gas sparging at 0.2 v v(-1) min(-1). Higher steady state cell densities were obtained on a medium containing maltose and yeast extract. In order to design a defined and minimal media, the nutritional requirements of T hydrothermalis were then investigated using continuous culture in the absence of elemental sulphur in the gas-lift bioreactor. First, the complex nutriments were replaced and a defined medium containing maltose, 19 amino acids and the two nitrogenous bases adenine and thymine, was determined. Secondly, selective feedings and withdrawal of amino acids showed requirements for 14 amino acids.
PY 2005
SO Research in Microbiology
SN 0923-2508
PU Elsevier
VL 156
IS 1
UT 000226425300010
BP 82
EP 87
DI 10.1016/j.resmic.2004.08.001
ID 365
ER

EF