FN Archimer Export Format PT J TI A novel mcl PHA-producing bacterium, Pseudomonas guezennei sp nov, isolated from a 'kopara' mat located in Rangiroa, an atoll of French Polynesia BT AF SIMON-COLIN, Christelle ALAIN, Karine COLIN, Sebastien COZIEN, Joelle COSTA, B GUEZENNEC, Jean RAGUENES, Gerard AS 1:1;2:2;3:1;4:1;5:;6:1;7:1; FF 1:PDG-DOP-DCB-BM-BMM;2:;3:PDG-DOP-DCB-BM-BMM;4:PDG-DOP-DCB-BM-BMM;5:;6:PDG-DOP-DCB-BM-BMM;7:PDG-DOP-DCB-BM-BMM; C1 IFREMER, Ctr Brest, BIOMAR BMM, F-29280 Plouzane, France. Equipe Ecol Microbienne Mrine, Lab Oceanog Biol Banyuls, Banyuls Sur Mer, France. C2 IFREMER, FRANCE LOMIC, FRANCE SI BREST SE PDG-DOP-DCB-BM-BMM PDG-DOP-DCB-EEP-LMEE IN WOS Ifremer jusqu'en 2018 copubli-france IF 2.028 TC 12 UR https://archimer.ifremer.fr/doc/2008/publication-3858.pdf LA English DT Article DE ;Pseudomonas;Polyhydroxyalkanaote;Microbial mat;Kopara AB Aims: The aim of the present study was to describe an aerobic, mesophilic and heterotrophic bacterium, designated RA26, able to produce a medium-chain-length polyhydroxyalkanoate (PHA). It was isolated from a French Polynesian bacterial mat located in the atoll of Rangiroa. Methods and Results: This micro-organism, on the basis of the phenotypical features and genotypic investigations can be clearly assigned to the Pseudomonas genus and the name of Pseudomonas guezennei is proposed. Optimal growth occurs between 33 and 37 degrees C, at a pH between 6 4 and 7 1 and at ionic strength of 15 g 1(-1) of sea salts. The G+C content of DNA is 63.2%. Under laboratory conditions, this bacterium produced a novel, medium-chain-length PHA, mainly composed of 3-hydroxydecanaote ( 64 mol.%) and 3-hydroxyoctanoate ( 24 mol.%) ( GC-MS, NMR) from a single nonrelated carbon substrate, i.e. glucose. Conclusions: The bacterium P. guezennei produces a novel PHA mcl with elastomeric properties. Significance and Impact of the Study: PHAs share physical and material properties that recommend them for application in various areas, and are considered as an alternative to nonbiodegradable plastics produced from fossil oils. In this study, we describe a new bacteria with the capability to synthesize a novel PHA with promising biotechnological applications. PY 2008 PD FEB SO Journal of Applied Microbiology SN 1364-5072 PU Blackwell science VL 104 IS 2 UT 000252496100027 BP 581 EP 586 DI 10.1111/j.1365-2672.2007.03568.x ID 3858 ER EF