FN Archimer Export Format
PT J
TI Continuous enrichment cultures: insights into prokaryotic diversity and metabolic interactions in deep-sea vent chimneys
BT 
AF POSTEC, Anne
   LESONGEUR, Francoise
   PIGNET, Patricia
   OLLIVIER, B
   QUERELLOU, Joel
   GODFROY, Anne
AS 1:1;2:1;3:1;4:2;5:1;6:1;
FF 1:PDG-DOP-DCB-EEP-LMEE;2:PDG-DOP-DCB-EEP-LMEE;3:PDG-DOP-DCB-EEP-LMEE;4:;5:PDG-DOP-DCB-EEP-LMEE;6:PDG-DOP-DCB-EEP-LMEE;
C1 IFREMER, Ctr Brest, Lab Microbiol Environm Extremes, UMR 6197, F-29280 Plouzane, France.
   Univ Aix Marseille 1, CESB ESIL, Lab IRD Microbiol Anaerobies, UR 101, F-13288 Marseille, France.
C2 IFREMER, FRANCE
   UNIV AIX MARSEILLE 1, FRANCE
SI BREST
SE PDG-DOP-DCB-EEP-LMEE
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.317
TC 20
TU Centre national de la recherche scientifique
   Institut français de recherche pour l'exploitation de la mer
   Université de Bretagne Occidentale
UR https://archimer.ifremer.fr/doc/2007/publication-3885.pdf
LA English
DT Article
CR ATOS
BO Unknown
DE ;Metabolic interactions;16S rRNA gene;Thermophiles;Bioreactor;Continuous enrichment cultures;Deep sea hydrothermal vent;Microbial diversity
AB The prokaryotic diversity of culturable thermophilic communities of deep-sea hydrothermal chimneys was analysed using a continuous enrichment culture performed in a gas-lift bioreactor, and compared to classical batch enrichment cultures in vials. Cultures were conducted at 60 degrees C and pH 6.5 using a complex medium containing carbohydrates, peptides and sulphur, and inoculated with a sample of a hydrothermal black chimney collected at the Rainbow field, Mid-Atlantic Ridge, at 2,275 m depth. To assess the relevance of both culture methods, bacterial and archaeal diversity was studied using cloning and sequencing, DGGE, and whole-cell hybridisation of 16S rRNA genes. Sequences of heterotrophic microorganisms belonging to the genera Marinitoga, Thermosipho, Caminicella (Bacteria) and Thermococcus (Archaea) were obtained from both batch and continuous enrichment cultures while sequences of the autotrophic bacterial genera Deferribacter and Thermodesulftitator were only detected in the continuous bioreactor culture. It is presumed that over time constant metabolite exchanges will have occurred in the continuous enrichment culture enabling the development of a more diverse prokaryotic community. In particular, CO2 and H-2 produced by the heterotrophic population would support the growth of autotrophic populations. Therefore, continuous enrichment culture is a useful technique to grow over time environmentally representative microbial communities and obtain insights into prokaryotic species interactions that play a crucial role in deep hydrothermal environments.
PY 2007
PD NOV
SO Extremophiles
SN 1431-0651
PU Springer
VL 11
IS 6
UT 000251578100001
BP 747
EP 757
DI 10.1007/s00792-007-0092-z
ID 3885
ER

EF