FN Archimer Export Format PT J TI Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers BT AF GAGNAIRE, Beatrice DUCHEMIN, M AUFFRET, Mathieu THOMAS-GUYON, Helene RENAULT, Tristan AS 1:1,2;2:3,4;3:3;4:2;5:1; FF 1:PDG-DOP-DCN-AGSAE-LGP;2:;3:PDG-DOP-DCB-PFOM-ARN;4:;5:PDG-DOP-DCN-AGSAE-LGP; C1 IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France. Ctr Rech Ecosyst Littoraux Anthropises, UMR 6217, F-17042 La Rochelle, France. Univ Bretagne Occidentale, Lab Sci Environm Marin, CNRS UMR 6539, Inst Univ European Mer, F-29280 Plouzane, France. Inst Natl Rech Sci Sante, Inst Armand Frappier, Pointe Claire, PQ H9R 1G6, Canada. C2 IFREMER, FRANCE CTR RECH ECOSYST LITTORAUX ANTHROPISES, FRANCE UBO, FRANCE INRS-IAF, CANADA SI LA TREMBLADE BREST UNIV-FRANCAISE SE PDG-DOP-DCN-AGSAE-LGP PDG-DOP-DCB-PFOM-ARN PDG-DOP-DCN-AGSAE-CRELA IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france copubli-int-hors-europe IF 0.667 TC 17 UR https://archimer.ifremer.fr/doc/2008/publication-3898.pdf LA English DT Article DE ;Crassostrea gigas;Phagocytosis;Hemocytes;Flow cytometry AB Parameters of hemocyte populations have been considered as relevant indicators of bivalve health and are currently used in immunotoxicological studies. Hemocytes in hemolymph can be collected by puncturing either the pericardial cavity or the adductor muscle sinus with a syringe. Flow cytometry is a methodological approach that is increasingly being used in laboratories for the study of hemocyte parameters in aquatic invertebrates. However, various protocols for hemocyte processing in laboratories equipped with different types of cytometers have been published. In this context, two flow cytometers ( EPICS XL4 (R), Beckman Coulter and FacsCalibur (R), Becton Dickinson) and two sites of hemocyte collection ( pericardial cavity and adductor muscle sinus) were compared for the analysis of hemocyte parameters in the Pacific oyster, Crassostrea gigas. Hemolymph cells were analyzed in terms of their number and organelle contents. Cell mortality, phagocytosis, non specific esterase, extension of the lysosomal compartment and production of reactive oxygen species were quantified. The results showed that the phagocytic index was higher for hemocytes obtained in the muscle sinus hemolymph. The results are discussed with respect to the potential use of flow cytometry as a tool for hemocyte studies in bivalves. PY 2008 SO Aquatic Living Resources SN 0990-7440 PU EDP Sciences VL 21 IS 1 UT 000254672000005 BP 39 EP 43 DI 10.1051/alr:2008009 ID 3898 ER EF