FN Archimer Export Format
PT J
TI Characterization of a gonad-specific transforming growth factor-beta superfamily member differentially expressed during the reproductive cycle of the oyster Crassostrea gigas
BT 
AF FLEURY, Elodie
   FABIOUX, Caroline
   LELONG, C
   FAVREL, P
   HUVET, Arnaud
AS 1:1,2;2:1,2;3:2;4:2;5:1;
FF 1:PDG-DOP-DCB-PFOM-PI;2:PDG-DOP-DCB-PFOM-PI;3:;4:;5:PDG-DOP-DCB-PFOM-PI;
C1 IFREMER, Ctr Brest, UMR M100 PE2M, F-29280 Plouzane, France.
   Univ Caen, UMR M100 PE2M, F-14032 Caen, France.
C2 IFREMER, FRANCE
   UNIV CAEN, FRANCE
SI BREST
SE PDG-DOP-DCB-PFOM-PI
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.578
TC 31
UR https://archimer.ifremer.fr/doc/2008/publication-3899.pdf
LA English
DT Article
DE ;Reproduction;Oyster;Gene expression;Transforming growth factor;Bivalve mollusc
AB Through differential screening between oyster families selected for high and low summer survival, we have characterized a new transforming growth factor-beta (TGF-beta) superfamily member. This novel factor, named oyster-gonadal-TGF beta-like (og-TGF beta-like), is synthesized as a 307 amino acid precursor and displays 6 of the 7 characteristic cysteine residues of the C-terminal, mature peptide. Sequence comparison revealed that og-TGF beta-like has a low percentage of identity with other known TGF-beta superfamily members, suggesting that og-TGF beta-like is a derived member of this large superfamily. Real-time PCR (RT-PCR) analysis in different oyster tissues showed that og-TGF beta-like is specifically expressed in both male and female gonads, at distinct levels according to the reproductive stage. Og-TGF beta-like relative expression was the lowest at the initiation of the reproductive cycle and increased as maturation proceeded to achieve a maximal level in fully mature female and male oysters. In situ hybridisation demonstrated that expression was exclusively detected in the somatic cells surrounding oocytes and spermatocytes. The role of this newly-characterized TGF beta member in the reproduction of cupped oyster is discussed in regard to the specificity and the localization of its expression, which singularly contrasts with the pleiotropic roles in a variety of physiological processes commonly ascribed to most TGF-beta family members identified so far. (C) 2007 Elsevier B.V. All rights reserved.
PY 2008
PD FEB
SO Gene
SN 0378-1119
PU Elsevier
VL 410
IS 1
UT 000254727200023
BP 187
EP 196
DI 10.1016/j.gene.2007.12.017
ID 3899
ER

EF