FN Archimer Export Format
PT J
TI Ecophysiological and metabolic adaptations to sulphide exposure of the oyster Crassostrea gigas
BT 
AF LE MOULLAC, Gilles
   CHEIZE, Marie
   GASTINEAU, Olivier
   DANIEL, Jean-Yves
   LE COZ, Jean-Rene
   HUVET, Arnaud
   MOAL, Jeanne
   POUVREAU, Stephane
   VAN WORMHOUDT, A
   SAMAIN, Jean-Francois
AS 1:1;2:2;3:2;4:2;5:2;6:2;7:2;8:1;9:3;10:2;
FF 1:PDG-DOP-DCOP-AQUAPOL-LDHP;2:PDG-DOP-DCB-PFOM-PI;3:PDG-DOP-DCB-PFOM-ARN;4:PDG-DOP-DCB-PFOM-PI;5:PDG-DOP-DCB-PFOM-PI;6:PDG-DOP-DCB-PFOM-PI;7:PDG-DOP-DCB-PFOM-PI;8:PDG-DOP-DCB-PFOM-PI;9:;10:PDG-DOP-DCB-PFOM-PI;
C1 IFREMER, UMR Physiol & Ecophysiol Mollusques Marins 100, F-29840 Argenton En Landunvez, France.
   IFREMER, Ctr Brest, UMR Physiol & Ecophysiol Mollusques Marins 100, F-29280 Plouzane, France.
   Museum Natl Hist Nat, Stn Biol Marine, UMR Biol Organismes & Ecosyst Marines 5178, F-29900 Concarneau, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   MNHN, FRANCE
SI TAHITI
   BREST
   ARGENTON
SE PDG-DOP-DCOP-AQUAPOL-LDHP
   PDG-DOP-DCB-PFOM-PI
   PDG-DOP-DCB-PFOM-ARN
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 0.576
TC 2
UR https://archimer.ifremer.fr/doc/2008/publication-3924.pdf
LA English
DT Article
DE ;ATP;AEC;Electron transport system;Succcinate;Alanine;Phosphoenolpyruvate carboxykinase;Pyruvate kinase;Oxygen consumption;Clearance rate;Crassostrea gigas;Sulphide;Oyster
AB The purpose of this study is to examine the response of the oyster Crassostrea gigas to sulphide at different biological scales. A first experiment was designed to measure for 20 h the clearance (CR) and oxygen consumption (OC) rates of oysters exposed at different concentrations of sulphide (0, 3, 6, 12, 20, and 40 mu M). The second experiment was carried out to evaluate the metabolic adaptations to chronic sulphide exposure for 10 days by measuring PK and PEPCK enzyme activities, succinate and alanine content, the adenylate energy charge (AEC), and the activity of the electron transport system (ETS)in whole oysters at above 20 mu M. PK and PEPCK mRNAs and enzyme activities have been measured in adductor muscle. When exposed to sulphide above 20 mu M, CR stopped, whereas oyster maintained their average OC rate between 1 and 2 mg O-2 h(-1) g(-1) dw. In the second experiment, sulphide exposure of oysters resulted in higher mortality, reduced the glycolytic flux by inhibiting the PK activity and decreased the ETS activity leading to a lower AEC. At day 10, a kind of recovery took place: the ETS activity and AEC of oysters exposed to sulphide increased to the level of the control oysters. In the adductor muscle, sulphide did not affect the transcriptional level of PK; PK was only regulated at enzyme level. Conversely, PEPCK was regulated only at transcriptional level. Nevertheless, muscle exhibited a wholly anaerobic metabolism by the significant increase of alanine and succinate contents followed by the significantly decrease of the ATP content during the 10 days of the experiment. This experiment showed that sulphide involved partially the whole oyster and wholly the adductor muscle in an anaerobic pathway of energy production; but oxygen was needed for the detoxification of the organism.
PY 2008
PD APR
SO Journal of Shellfish Research
SN 0730-8000
PU National Shellfisheries Association
VL 27
IS 2
UT 000254880600015
BP 355
EP 363
ID 3924
ER

EF