FN Archimer Export Format
PT J
TI The effect of environmental salinity on the proteome of the sea bass (Dicentrarchus labrax L.)
BT 
AF KY, Chin Long
   DE LORGERIL, Julien
   HIRTZ, C
   SOMMERER, N
   ROSSIGNOL, M
   BONHOMME, F
AS 1:1;2:1;3:3;4:4;5:4;6:2;
FF 1:PDG-DOP-DCM-BOME-LALR;2:PDG-DOP-DCM-BOME-LALR;3:;4:;5:;6:;
C1 IFREMER, F-34250 Palavas Les Flots, France.
   IFREMER, CNRS, UM2, UMR 5171, F-34095 Montpellier 5, France.
   Univ Montpellier 1, Lab Physiol & Endocrinol Orofaciale, F-34193 Montpellier 5, France.
   INRA, UR 1199, Lab Proteom, F-34060 Montpellier 1, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   UNIV MONTPELLIER, FRANCE
   INRA, FRANCE
SI PALAVAS
   MONTPELLIER
SE PDG-DOP-DCM-BOME-LALR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-univ-france
IF 2.64
TC 13
UR https://archimer.ifremer.fr/doc/2007/publication-3952.pdf
LA English
DT Article
DE ;Two dimensional gel electrophoresis;Sea bass;Salinity;Real time PCR;MALDI TOF mass spectrometry
AB The European sea bass, Dicentrarchus labrax L., tolerates a range of salinities from freshwater to hyper-saline. To study differences in protein expression, fish were reared in both freshwater and seawater. After 3-month acclimation, gill and intestine epithelia were collected and the soluble protein extracted. In all, 362 spots were differentially expressed in the gills and intestines of fishes reared in seawater compared to those from freshwater. Fifty differential protein spots were excised from a colloidal Coomassie-stained gel. Nine separate protein spots were identified unambiguously by mass spectrometry and database searching. Among the six proteins over-expressed in gill cells in seawater, five were cytoskeleton proteins and one was the aromatase cytochrome P450. In gill cells under freshwater conditions, the two over-expressed proteins identified were the prolactin receptor and the major histocompatibility complex class II beta-antigen. In intestinal cells under freshwater conditions, the Iroquois homeobox protein Ziro5 was upregulated over ninefold. The expression of these proteins, their possible direct or indirect roles in the adaptation of D. labrax to salinity, and their correspondences with a previous study are discussed.
PY 2007
PD DEC
SO Animal Genetics
SN 0268-9146
PU Blackwell science
VL 38
IS 6
UT 000251327600008
BP 601
EP 608
DI 10.1111/j.1365-2052.2007.01652.x
ID 3952
ER

EF